1887

Abstract

SUMMARY: The level of glutamic acid concentration measured inside the streptococcal cell during assimilation represents a balance between the rate at which the amino-acid is withdrawn from the external medium and the rate at which it is metabolized within the cell.

Treatment of the cells, prior to or during assimilation, with dyes of the triphenylmethane series results in raising the level of free glutamic acid attained within the cell. Evidence is presented that the triphenylmethane dyes prevent the metabolism of glutamic acid within the cell. The activity of the triphenylmethane molecule as an antibacterial agent and in raising the level of glutamic acid assimilation is increased by alkyl substitution and can be correlated with the lipid solubility of the dye.

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/content/journal/micro/10.1099/00221287-1-3-299
1947-09-01
2021-07-29
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References

  1. Angier R. B., Boothe J. H., Hutchings B. L., Mowat J. H., Semb J., Stokstad E. L. R., Subbarow Y., Waller C. W., Cosulich D. B., Fahrenbach M. J., Hultquist M. E., Kuh E., Northey E. H., Seeger D. R., Sickels J. P., Smith J. M. Jr 1946; The structure and synthesis of the liver L. casei factor. Science 103:667
    [Google Scholar]
  2. Freeland J., Gale E. F. 1947; The amino-acid composition of certain bacteria and yeasts. Biochem. J 41:135
    [Google Scholar]
  3. Gale E. F. 1945; Studies on bacterial amino-acid decarboxylases, 5. The use of specific decarboxylase preparations in the estimation of amino-acids and in protein analysis. Biochem. J 39:46
    [Google Scholar]
  4. Gale E. F. 1947a; The assimilation of amino-acids by bacteria. 1. The passage of certain amino-acids across the cell wall and their concentration in the internal environment of Streptococcus faecalis. J. gen. Microbiol 1:53
    [Google Scholar]
  5. Gale E. F. 1947b; The assimilation of amino-acids by bacteria. 6. The effect of protein synthesis on glutamic acid accumulation and the action thereon of sulphathiazole. J. gen. Microbiol 1:327
    [Google Scholar]
  6. Gale E. F., Taylor E. S. 1947; The assimilation of amino-acids by bacteria. 5. The action of penicillin in preventing the assimilation of glutamic acid by Staphylococcus aureus. J. gen. Microbiol 1:314
    [Google Scholar]
  7. Hanby W. E., Rydon H. N. 1946; The capsular substance of Bacillus anthracis. Biochem. J 40:297
    [Google Scholar]
  8. Harvey E. N., Danielli J. F. 1938; Properties of the cell surface. Biol. Rev 13:319
    [Google Scholar]
  9. Kligler I. J. 1918; A study of the antiseptic properties of certain organic compounds. J. exp. Med 27:463
    [Google Scholar]
  10. Lichstein H. C., Cohen P. P. 1945; Transamination in bacteria. J. biol. Chem 157:85
    [Google Scholar]
  11. Lichstein H. C., Gunsalus I. C., Umbreit W. W. 1945; Function of the vitamin B6 group: pyridoxal phosphate (codecarboxylase) in transamination. J. biol. Chem 161:311
    [Google Scholar]
  12. Mccalla T. M. 1941; The reaction of certain stains with bacteria. Stain. Tech 16:27
    [Google Scholar]
  13. Mccalla T. M. 1942; Uptake of ions by bacteria. Chem. Abstr 36:1978
    [Google Scholar]
  14. Mccalla T. M., Clark F. E. 1941; Dye absorption by bacteria at various H-ion concentrations. Stain. Tech 16:95
    [Google Scholar]
  15. Marshall E. K. Jr Lockwood J. S., Dubos R. J. 1941 Chemotherapy pp. 29–42 Univ. Pennsylvania Press;
    [Google Scholar]
  16. Ratner S., Blanchard M., Green D. E. 1946; Isolation of a peptide of p-aminobenzoic acid from yeast. J. biol. Chem 164:691
    [Google Scholar]
  17. Schulman J. H. 1937; Structure in relation to living biological functions. Trans. Faraday Soc 33:1116
    [Google Scholar]
  18. Stearn A. E. 1933; Rationalization, from the viewpoint of physical chemistry, of the behaviour of bacteria towards dyes, with special reference to staining. J. Bact 25:21
    [Google Scholar]
  19. Stearn A. E., Stearn E. W. 1930; Chemotherapeutic equilibria. J. exp. Med 51:341
    [Google Scholar]
  20. Stearn E. W. 1927; The correlation of variation in dye sensitivity with Gram character in certain Gram-positive organisms. J. Bact 14:349
    [Google Scholar]
  21. Stearn E. W., Stearn A. E. 1924; The chemical mechanism of bacterial behaviour. 1. Behaviour towards dyes; factors controlling the Gram reaction; 2. A new theory of the Gram reaction; 3. The problem of bacteriostasis. J. Bact 9:463–479 491
    [Google Scholar]
  22. Stearn E. W., Stearn A. E. 1925; A study of the chemical differentiation of bacteria. J. Bact 10:13
    [Google Scholar]
  23. Stearn E. W., Stearn A. E. 1926; Conditions and reactions defining dye bacteriostasis. J. Bact 11:345
    [Google Scholar]
  24. Taylor E. S. 1947; The assimilation of amino-acids by bacteria. 3. Concentration of free amino-acids in the internal environment of various bacteria and yeasts. J. gen. Microbiol 1:86
    [Google Scholar]
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