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Volume 1, Issue 2

Methods of Penicillin Production in Submerged Culture on a Pilot-Plant Scale Free

Abstract

This paper gives details of a 50 gal. fermentation vessel designed for investigating the formation of antibiotics (or other metabolic products) by microorganisms grown in submerged culture. This vessel has been used for investigating the submerged culture production of penicillin by X 1612 and Q176, and certain results relating to the size of the inoculum and the yields obtainable from these strains in synthetic and other media have been obtained. Culture fluids containing from 400 to 500 Oxford units penicillin/ml. have been obtained with cultures of Q176 in a corn-steep liquor medium.

A method of extracting penicillin from the broth has been worked out, based on solvent transfer, the method being applicable on virtually any scale of operation and involving only relatively simple equipment. It has the advantage of reducing the time of contact of penicillin with acid to such a degree that extraction at room temperature is possible, although extraction at still lower temperatures improves the yield. Using this method of extraction we have obtained calcium penicillin with a potency of 940 Oxford units/mg., the overall recovery from the broth being of the order of 35–50%.

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© Society for General Microbiology, 1947
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1947-06-01
2024-09-19
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References

  1. Appleby J. C., Knowles E., Mcallister R. C. A., Pearson J., White T. 1947; The production of tyrothricin by submerged culture of Bacillus brevis in synthetic media. J. gen. Microbiol 1:145
     [Google Scholar]
  2. Foster J. W., Woodruff H. B., Mcdaniel L. E. 1946; Microbiological aspects of penicilin. IV. Production of penicillin in submerged cultures of Penidllium notatum. J. Bact 51:465
     [Google Scholar]
  3. Foster J. W., Woodruff H. B., Perlman D., Mcdaniel L. E., Wilker B. L., Hendlin D. 1946; Microbiological aspects of penicillin. IX. Cotton seed meal as a substitute for corn-steep liquor in penicillin production. J. Bact 51:695
     [Google Scholar]
  4. Grenfell E., Legge B. J., White T. 1947; Microbiological aspects of the submerged culture production of penicillin on a pilot-plant scale. J. gen. Microbiol 1:171
     [Google Scholar]
  5. Knight S. G., Frazier W. C. 1945; The control of contaminants in penicillin fermentations by antiseptic chemicals. J. Bact 50:505
     [Google Scholar]
  6. Koffler H., Emerson A. L., Perlman D., Burris R. H. 1945; Chemical changes in submerged penicillin fermentations. J. Bact 50:517
     [Google Scholar]
  7. Koffler H., Knight S. G., Emerson A. L., Burris A. H. 1945; The effect of certain chemicals on penicillin production and mould metabolism in shake flask fermentations. J. Bact 50:549
     [Google Scholar]
  8. Moyer A. J., Coghill R. D. 1946a; Penicillin. VIII. Production of penicillin in surface cultures. J. Bact 51:57
     [Google Scholar]
  9. Moyer A. J., Coghill R. D. 1946b; Penicillin. IX. The laboratory scale production of penicillin in submerged culture by Penicillium notatum Westling (NRRL 832). J. Bact 51:79
     [Google Scholar]
  10. Raper K. B., Fennell D. I. 1946; The production of penicillin X in submerged culture. J. Bact 51:761
     [Google Scholar]
  11. Raper K. B., Alexander D. F., Coghill R. D. 1944; Penicillin. II. Natural variation and penicillin production in Penicillium notatum and allied species. J. Bact 48:639
     [Google Scholar]
  12. Schaffer P. A., Hartman A. F. 1920; The iodometric determination of copper and its use in sugar analysis. II. Methods for the determination of reducing sugars in blood, urine, milk and other solutions. J. biol. Chem 45:465
     [Google Scholar]
  13. Stefaniak J. J., Gailey F. B., Brown C. S., Johnson M. J. 1946; Pilot-plant equipment for submerged production of penicillin. Ind. Engng Chem 38:666
     [Google Scholar]
/content/journal/micro/10.1099/00221287-1-2-187
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Methods of Penicillin Production in Submerged Culture on a Pilot-Plant Scale
Microbiology 1, 187 (1947); https://doi.org/10.1099/00221287-1-2-187
/content/journal/micro/10.1099/00221287-1-2-187
/content/journal/micro/10.1099/00221287-1-2-187
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