1887

Abstract

SUMMARY: The use of mechanical air filtration combined with ultra-violet irradiation of the filtered air has aided markedly in avoiding contamination by undesirable micro-organisms during pilot-plant scale work on penicillin production. By maintaining master cultures of strains in sterile soil it has been found possible to eliminate strain variation and to maintain the penicillin-producing capacity of the strains. This procedure, followed by transfer of the organism to a rye grain substrate, gives a convenient method of securing large numbers of spores for large-scale work.

It has been found to be desirable to check the purity of the strains, their penicillin-producing capacity and the viability of the spores at various stages of the production sequence; methods have been developed for each of these requirements. Improvements in the cup assay method of estimating penicillin have eliminated certain difficulties encountered in this assay and have enabled the overall error of the method to be reduced to 5%. A colorimetric method of assay has been developed which gives results agreeing with those of the cup assay. With this new method the penicillin content of a sample can be estimated in 5 hr.

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/content/journal/micro/10.1099/00221287-1-2-171
1947-06-01
2021-10-20
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References

  1. Abraham E. P., Chain E., Fletcher C. M., Gardner D. A., Heatley N. G., Jennings M. A., Florey H. W. 1941; Further observations on penicillin. Lancet ii:177
    [Google Scholar]
  2. Appleby J. C., Knowles E., Mcallister R. C. A., Pearson J., White T. 1947; The production of tyrothricin by submerged culture of Bacillus brevis in synthetic media. J. gen. Microbiol 1:145
    [Google Scholar]
  3. Appleby J. C., Knowles E., Pearson J., White T. 1947; A preliminary study of the formation, assay and stability of tyrothricin. J. gen. Microbiol 1:137
    [Google Scholar]
  4. Beadle G. W., Mitchell H. K., Bonner D. 1945; Improvements in the cylinder-plate method for penicillin assay. J. Bact 49:101
    [Google Scholar]
  5. Clutterbuck P. W., Lovell R., Raistrick H. 1932; Studies in the biochemistry of micro-organisms. XXVI. The formation from glucose by members of the Penicillium chrysogenum series of a pigment, an alkali-soluble protein and penicillin-the antibacterial substance of Fleming. Biochem. J 26:1907
    [Google Scholar]
  6. Fleming A. 1929; On the antibacterial action of cultures of a Penicillium, with special reference to their use in the isolation of B. influenzae. Brit. J. exp. Path 10:226
    [Google Scholar]
  7. Foster J. W., Woodruff H. B. 1943; Microbiological aspects of penicillin. I. Methods of assay. J. Bact 46:187
    [Google Scholar]
  8. Foster J. W., Woodruff H. B. 1944; Microbiological aspects of penicillin. VI. Procedure for the cup assay for penicillin. J. Bact 47:43
    [Google Scholar]
  9. Foster J. W., Woodruff H. B., Mcdaniel L. E. 1943; Microbiological aspects of penicillin. III. Production of penicillin in surface cultures of Penicillium notatum. J. Bact 46:421
    [Google Scholar]
  10. Gordon J. J., Grenfell E., Knowles E., Legge B. J., Mcallister R. C. A., White T. 1947; Methods of penicillin production in submerged culture on a pilot-plant scale. J. gen. Microbiol 1:187
    [Google Scholar]
  11. Hanson H. J., Myers W. G., Stahly G. L., Birkeland J. M. 1946; Variation in Penicillium notatum induced by the bombardment of spores with neutrons. J. Bact 51:9
    [Google Scholar]
  12. Knudsen L. F., Randall W. A. 1945; Penicillin assay and its control chart analysis. J. Bact 50:187
    [Google Scholar]
  13. Koffler H., Emerson A. L., Perlman D., Burris R. M. 1945; Chemical changes in submerged penicillin fermentations. J. Bact 50:517
    [Google Scholar]
  14. Moyer A. J., Coghill R. D. 1946a; Penicillin. VIII. Production of penicillin in surface cultures. J. Bact 51:57
    [Google Scholar]
  15. Moyer A. J., Coghill R. D. 1946b; Penicillin. IX. The laboratory scale production of penicillin in submerged cultures by Penicillium notatum Westling (NRRL 832). J. Bact 51:79
    [Google Scholar]
  16. Rahn O. 1945; Physical methods of sterilization of micro-organisms. Bact. Rev 9:1
    [Google Scholar]
  17. Raper K. B., Alexander D. F., Coghill R. D. 1944; Penicillin. II. Natural variation and penicillin production in Penicillium notatum and allied species. J. Bact 48:639
    [Google Scholar]
  18. Raper K. B., Fennel D. I. 1946; The production of penicillin X in submerged culture. J. Bact 51:761
    [Google Scholar]
  19. Reyniers J. A. 1943 Micrurgical and Germ-free Methods Baltimore: Thomas;
    [Google Scholar]
  20. Schmidt W. M., Moyer A. J. 1944; Penicillin. I. Methods of assay. J. Bact 47:199
    [Google Scholar]
  21. Thom C., Raper K. B. 1945 A Manual of the Aspergilli Baltimore: Williams and Wilkins;
    [Google Scholar]
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