SUMMARY: The organisms whose antibacterial powers are to be investigated are grown in parallel streaks on cellophan superposed on an agar plate. The cellophan is stripped off and the test organisms are streaked on the sterile agar surface thereby revealed, at right angles to the first streaks. After incubation, growth of the test organisms will be interrupted in those areas in which an inhibitor has diffused through the cellophan from the first organisms. There are some limitations in the application of the method.


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  1. Dubos R. J. 1939; Studies on a bactericidal agent extracted from a soil bacillus. I.Preparation of the agent. Its activity in vitro. J. exp. Med 70:1
    [Google Scholar]
  2. Gratia A. 1944; Application de la culture sur cellophane à l’étude et à la production des antagonismes microbiens. C.R. Soc. Biol Paris: 138893
    [Google Scholar]
  3. Heatley N. G., Florey H. W. 1946; An antibiotic from Bacterium coli. Brit. J. exp. Path 27:378
    [Google Scholar]
  4. Oxford A. E. 1944; Diplococcin, an antibacterial protein elaborated by certain milk streptococci. Biochem. J 38:178
    [Google Scholar]
  5. Seymour W. B. 1940; The preparation of cellophane membranes of graded permeability. J. biol. Chem 134:701
    [Google Scholar]

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