1887

Abstract

SUMMARY: Strain selection and the addition of yeast extract to the medium have enabled high yields of tyrothricin to be produced from the culture fluids of Contrary to the accepted view, this organism does ferment carbohydrates with the production of acid, and a description of the characters of the organism is given. A method of assaying tyrothricin has been developed and used to investigate the stability of aqueous solutions of the material. Tyrothricin produced in aerated submerged culture appears to be more stable in solution than that produced by surface culture.

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/content/journal/micro/10.1099/00221287-1-2-137
1947-06-01
2021-07-24
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References

  1. Appleby J. C., Knowles E., Mcallister R. C. A., Pearson J., White T. 1947; The production of tyrothricin by submerged culture of Bacillus brevis in synthetic media. J. gen. Microbiol 1:145
    [Google Scholar]
  2. Bergey D. H., Breed R. S., Murray E. G. D., Hitchens A. P. 1939; Bergey’s Manual of Determinative Bacteriology. , 5th ed.. London: Baillière, Tindall and Cox;
    [Google Scholar]
  3. Dimick K. P. 1943; A quantitative method for the determination of tyrothricin. J. biol. Chem 149:387
    [Google Scholar]
  4. Dubos R. J. 1939a; Studies on a bactericidal agent extracted from a soil bacillus. I. Preparation of the agent. Its activity in vitro. J. exp. Med 70:1
    [Google Scholar]
  5. Dubos R. J. 1939b; Studies on a bactericidal agent extracted from a soil bacillus.II. Protective effect of the bactericidal agent against experimental pneumococcus infection in mice. J. exp. Med 70:11
    [Google Scholar]
  6. Dubos R. J. 1939C; Bactericidal effect of an extract of a soil bacillus on Grampositive cocci. Proc. Soc. exp. Biol., N.Y 40:311
    [Google Scholar]
  7. Dubos R. J. 1940; The effect of specific agents extracted from soil micro-organisms upon experimental bacterial infections. Ann. intern. Med 13:2025
    [Google Scholar]
  8. Dubos R. J. 1941; Bacteriostatic and bactericidal agents obtained from saprophytic micro-organisms. J. Pediat 19:588
    [Google Scholar]
  9. Dubos R. J., Cattaneo C. 1939; Studies on a bactericidal agent extracted from a soil bacillus. III. Preparation and activity of a protein-free fraction. J. exp. Med 70:249
    [Google Scholar]
  10. Dubos R. J., Hotchkiss R. D. 1941; The production of bactericidal substances by aerobic sporulating bacilli. J. exp. Med 73:629
    [Google Scholar]
  11. Dubos R. J., Hotchkiss R. D. 1942; The origin, nature, and properties of gramicidin and tyrocidine. Trans. Coll. Phys. Philad 10:11
    [Google Scholar]
  12. Dubos R. J., Hotchkiss R. D., Coburn A. F. 1942; The effect of gramicidin and tyrocidine on bacterial metabolism. J. biol. Chem 146:421
    [Google Scholar]
  13. Gause G. F., Brahznikova M. G. 1944; Gramicidin S. Origin and mode of action. Lancet ii:715
    [Google Scholar]
  14. Hotchkiss R. D. 1941; The chemical nature OF gramicidin and tyrocidine. J. biol. Chem 141:170
    [Google Scholar]
  15. Hotchkiss R. D. 1944; Gramicidin, tyrocidine and tyrothricin. Advances in Enzymology 4153 New York: Interscience Publishers;
    [Google Scholar]
  16. Hotchkiss R. D., Dubos R. J. 1940a; Fractionation of the bactericidal agent from cultures of a soil bacillus. J. biol. Chem 132:791
    [Google Scholar]
  17. Hotchkiss R. D., Dubos R. J. 1940b; Chemical properties of bactericidal substances isolated from cultures of a soil bacillus. J. biol. Chem 132:793
    [Google Scholar]
  18. Hotchkiss R. D., Dubos R. J. 1940C; Bacterial fractions from an aerobic sporulating bacillus. J. biol. Chem 136:803
    [Google Scholar]
  19. Hotchkiss R. D., Dubos R. J. 1941; The isolation of bactericidal substances from cultures of Bacillus brevis. J. biol. Chem 141:155
    [Google Scholar]
  20. Landy M., Dicken D. M., Bicking M. M., Mitchell W. R. 1942; Use of avidin in studies on biotin requirements of micro-organisms. Proc. Soc. exp. Biol., N.Y 49:441
    [Google Scholar]
  21. Lewis J. C., Dimick K. P., Feustel I. C. 1945; Production of tyrothricin in cultures of Bacillus brevis. Industr. Engng Chem 37:996
    [Google Scholar]
  22. Stokes J. L., Woodward C. R. 1943; The formation of tyrothricin in submerged cultures of Bacillus brevis. J. Bact 46:83
    [Google Scholar]
  23. Tishler M., Stokes J. L., Trenner N. R., Conn J. B. 1941; Some properties of gramicidin. J. biol. Chem 141:197
    [Google Scholar]
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