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Microbial Genomics logo Microbial Genomics

Volume 11, Issue 11

Gene dependence during mammalian pneumonia and septicaemia infections Open Access

Abstract

With a limited number of traditional virulence factors, the success of the nosocomial pathogen is largely attributed to its ability to persist and resist. The niches encountered during infection vary significantly from the more commonly studied laboratory setting, and consequently, the genes responsible for pathogenesis have yet to be fully elucidated. This study utilized the AB5075-UW transposon mutant library with unbiased genome-wide transposon sequencing to identify the genetic basis for survival and fitness during pneumonia and septicaemia infections. We identified 128 genes essential for in-host survival, including 22 required for survival in all tissues. Additionally, 302 genes with significantly altered fitness were also identified. Tissue specificity was observed, highlighting the importance of genes associated with biosynthesis in the lungs, cell shape and structure in the kidneys and metal acquisition during septicaemia. The majority (89%) of the genes with aberrant fitness were constituents of the core genome. The findings were validated using a subset of targeted mutants, including those required for infection (, and ) or specifically septicaemia (, and ) or pneumonia (, and ), confirming that these observations were a result of specific fitness defects rather than aberrant growth. Taken together, these data provide the first global profile of genes required for fitness of during different disease states and growth in different tissues.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter baumannii , functional genomics , pneumonia , septicaemia , tissue tropism and Tn-Seq
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 82072344)
    • Principal Award Recipient: YingFu
  • Australian Research Council (Award DE200101524)
    • Principal Award Recipient: FrancescaShort
  • National Health and Medical Research Council (Award 2003416)
    • Principal Award Recipient: AntonY Peleg
  • National Health and Medical Research Council (Award APP1124917)
    • Principal Award Recipient: AntonY Peleg
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Gene dependence during mammalian Acinetobacter baumannii pneumonia and septicaemia infections
M Gen 11, 001556 (2025); https://doi.org/10.1099/mgen.0.001556
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