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Volume 11, Issue 4

Isolation, complete characterization and phylogeography of the first bacteriophage against , which encodes a pyruvate phosphate dikinase and represents a novel viral family Open Access

Abstract

are widely distributed in aquatic environments and are major pathogens commonly found in aquaculture environments, playing a significant role in human production activities and maintaining ecological stability. Here, a novel phage, vB_VneS_J26, which infects , was isolated from coastal seawater in Qingdao, China. Transmission electron microscopy revealed that vB_VneS_J26 exhibits siphovirus morphotype, with a linear double-stranded DNA genome of 82,477 bp in length and G+C content of 45.11 mol%, encoding 122 putative ORFs. Three auxiliary metabolic genes related to carbon metabolism and host cell redox processes were identified, including a pyruvate phosphate dikinase, which catalyses the reversible conversion between phosphoenolpyruvate and pyruvate and is rarely detected in viruses. Whole-genome phylogenetic and comparative genomic analyses suggested that vB_VneS_J26 represents a potential novel viral family, comprising six isolated vibriophages, proposed as . Phylogeographic analysis indicated that is primarily distributed in epipelagic and mesopelagic zones of the Arctic and temperate tropical oceans.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): comparative genomic analysis , phylogenetic analysis , phylogeography , pyruvate phosphate dikinase and vibriophage
Funding
This study was supported by the:
  • Fundamental Research Funds for the Central Universities (Award 202172002, 201812002, 202072001)
    • Principal Award Recipient: MinWang
  • Natural Science Foundation of China (Award 42120104006, 42176111)
    • Principal Award Recipient: MinWang
  • Key Technologies Research and Development Program (Award 2022YFC2807500)
    • Principal Award Recipient: MinWang
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2025-04-28
2026-02-07

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/content/journal/mgen/10.1099/mgen.0.001403
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Isolation, complete characterization and phylogeography of the first bacteriophage against Vibrio neocaledonicus, which encodes a pyruvate phosphate dikinase and represents a novel viral family
M Gen 11, 001403 (2025); https://doi.org/10.1099/mgen.0.001403
/content/journal/mgen/10.1099/mgen.0.001403
/content/journal/mgen/10.1099/mgen.0.001403
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