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Abstract

In 2013, foodborne outbreaks in Japan were linked to non-O1, non-O139 . However, laboratory tests have detected several serogroups, making it difficult to determine the causative agent. Therefore, whole-genome analyses revealed that only serogroup O144 possesses a genomic island with a type III secretion system (T3SS). A T3SS-deficient mutant was subsequently generated, and its pathogenicity was assessed using a rabbit ileal loop test. This led to the conclusion that serogroup O144 with T3SS was the causative agent of foodborne outbreaks. This study provides an illustrative example of the utilization of whole-genome data for pathogenicity and molecular epidemiological analyses in outbreak investigations.

Funding
This study was supported by the:
  • Japan Agency for Medical Research and Development (Award JP23fk0108663)
    • Principal Award Recipient: HidemasaIzumiya
  • Japan Agency for Medical Research and Development (Award JP20wm0125006, JP233fa627004, JP22wm0225023)
    • Principal Award Recipient: ToshioKodama
  • Nagasaki University (Award 2021-Ippan-02, 2022-Ippan-06, 2023-Ippan-06)
    • Principal Award Recipient: MasatomoMorita
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/mgen/10.1099/mgen.0.001364
2025-02-26
2026-01-21

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