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Volume 11, Issue 1

and infection in led to species-specific regulatory responses in the host and pathogen Open Access

Abstract

In recent decades, has surpassed as the leading cause of shigellosis, possibly due to species-specific differences in their transcriptomic responses. This study used dual RNA sequencing to analyse the transcriptomic responses of and the two species at early (10 minutes) and late (24 hours) stages of infection. While the nematode defence response was downregulated during both infections, only infection by led to downregulation of sphingolipid metabolism, cadmium ion response and xenobiotic response in . Furthermore, upregulates biofilm formation and energy generation/conservation during infection, acid resistance-related genes and biofilm regulators compared to . These findings highlight species-specific responses during infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Caenorhabditis elegans , dual-RNA sequencing , infection , Shigella flexneri and Shigella sonnei
Funding
This study was supported by the:
  • Monash University Malaysia High Impact Research Support Fund Award 2022 (Award STG000174)
    • Principal Award Recipient: BaoChi Wong
  • Ministry of Higher Education Malaysia Fundamental Research Grant Scheme (Award FRGS/1/2020/STG03/MUSM/03/1)
    • Principal Award Recipient: HockSiew Tan
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-01-24
2026-02-07

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/content/journal/mgen/10.1099/mgen.0.001339
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Shigella sonnei and Shigella flexneri infection in Caenorhabditis elegans led to species-specific regulatory responses in the host and pathogen
M Gen 11, 001339 (2025); https://doi.org/10.1099/mgen.0.001339
/content/journal/mgen/10.1099/mgen.0.001339
/content/journal/mgen/10.1099/mgen.0.001339
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