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Volume 10, Issue 11

Uncovering the transcriptome-wide RNA modifications in Open Access

Abstract

Despite being a major human pathogen, limited studies have reported RNA modifications in . These post-transcriptional modifications play crucial regulatory roles in bacteria and have also been shown to modulate bacterial virulence. Using nanopore sequencing, we characterized RNA modifications in a virulent strain (Ab-C98) under free-living (mid-exponential phase culture) and during an early stage of infection (3 h post-infection) in larvae. Analysis revealed that mC methylations are essential for ribosome synthesis, while mA and Ψ are involved in metabolic pathways and translation processes. Iron-chelating genes (mC and mA) and (mA and Ψ) and RNA polymerase subunit (mA and Ψ) were selectively modified during infection. This first transcriptome-wide study highlights the potential regulatory roles of mC, mA and Ψ modifications in during infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter baumannii , direct RNA sequencing , Galleria mellonella , infection and RNA modifications
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-11-20
2024-12-09
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Uncovering the transcriptome-wide RNA modifications in Acinetobacter baumannii
M Gen 10, 001327 (2024); https://doi.org/10.1099/mgen.0.001327
/content/journal/mgen/10.1099/mgen.0.001327
/content/journal/mgen/10.1099/mgen.0.001327
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