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Volume 10, Issue 11

Pangenomic insights into evolution and acquisition of functional genes for bioremediation Open Access

Abstract

is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the cassette, just downstream of its active RDase. A closed genome of this DCM-mineralizing lineage has previously evaded assembly. Here, we present the genomes of two novel strains, each of which was assembled from the metagenome of a distinct subculture from SC05. A pangenomic analysis of the genus, including RDase synteny and phylogenomics, reveals at least five species of based on average nucleotide identity, RDase and core gene synteny, as well as differential functional genes. An integration hotspot is also pinpointed in the genome, in which many recombinase islands have accumulated. This nested recombinase island encodes the active RDase and cassette in both SC05 genomes, indicating the transfer of key functional genes between species of . Horizontal gene transfer between these two novel strains has implications for the evolutionary history within the SC05 subcultures and of the genus as a whole, especially regarding adaptation to anthropogenic chemicals.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bioremediation , Dehalobacter , pangenome and reductive dehalogenase
Funding
This study was supported by the:
  • Canada Research Chairs
    • Principle Award Recipient: ElizabethA. Edwards
  • Canada Research Chairs
    • Principle Award Recipient: RadhakrishnanMahadevan
  • Genome Canada (Award 285MPR)
    • Principle Award Recipient: RadhakrishnanMahadevan
  • Genome Canada (Award 285MPR)
    • Principle Award Recipient: ElizabethA. Edwards
  • Natural Science and Engineering Research Council (NSERC) (Award Canada Graduate Scholarship)
    • Principle Award Recipient: OliviaBulka
  • Natural Science and Engineering Research Council (NSERC) (Award Discovery grant)
    • Principle Award Recipient: ElizabethA. Edwards
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2024-12-09
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Pangenomic insights into Dehalobacter evolution and acquisition of functional genes for bioremediation
M Gen 10, 001324 (2024); https://doi.org/10.1099/mgen.0.001324
/content/journal/mgen/10.1099/mgen.0.001324
/content/journal/mgen/10.1099/mgen.0.001324
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