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Microbial Genomics logo Microbial Genomics

Volume 10, Issue 10

Applying nanopore sequencing technology in sp.: a high-quality DNA isolation method for next-generation genomic studies Open Access

Abstract

Paracoccidioidomycosis is a severe systemic endemic mycosis caused by spp. which mainly affects individuals in Latin America. Progress in genomics has been slow, as evidenced by the incomplete reference databases available. Next-generation sequencing is a valuable tool for epidemiological surveillance and genomic characterization. With the ability to sequence long reads without the need for prior amplification, Oxford Nanopore Technology (ONT) offers several advantages, but high-quality and high-quantity DNA samples are required to achieve satisfactory results. Due to the low concentration of DNA in clinical samples and inefficient culture isolation methods, DNA extraction can be a significant barrier to genomic studies of this genus. This study proposes a method to obtain a high-coverage genome assembly for using an improved DNA extraction method suitable for sequencing with ONT. The assembly obtained was comparable in size to those constructed from available data from Illumina technology. To our knowledge, this is the first genome assembly of sp. of such a large size constructed using ONT.

  • Received:
  • Accepted:
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Keyword(s): assembly , nanopore , Paracoccidioides and sequencing
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2024-10-21
2025-11-10

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Applying nanopore sequencing technology in Paracoccidioides sp.: a high-quality DNA isolation method for next-generation genomic studies
M Gen 10, 001302 (2024); https://doi.org/10.1099/mgen.0.001302
/content/journal/mgen/10.1099/mgen.0.001302
/content/journal/mgen/10.1099/mgen.0.001302
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