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Microbial Genomics logo Microbial Genomics

Volume 10, Issue 6

GROND: a quality-checked and publicly available database of full-length 16S-ITS-23S rRNA operon sequences Open Access

Abstract

Sequence comparison of 16S rRNA PCR amplicons is an established approach to taxonomically identify bacterial isolates and profile complex microbial communities. One potential application of recent advances in long-read sequencing technologies is to sequence entire rRNA operons and capture significantly more phylogenetic information compared to sequencing of the 16S rRNA (or regions thereof) alone, with the potential to increase the proportion of amplicons that can be reliably classified to lower taxonomic ranks. Here we describe (enome-derived ibosomal peroatabase), a publicly available database of quality-checked 16S-ITS-23S rRNA operons, accompanied by multiple taxonomic classifications. will aid researchers in analysis of their data and act as a standardised database to allow comparison of results between studies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): amplicons , database , long read sequencing , microbiome , nanopore and rRNA
Funding
This study was supported by the:
  • Teagasc (Award 2020018)
    • Principle Award Recipient: MeghanaSrinivas
  • Science Foundation Ireand (Award SFI/12/RC/2273_P2)
    • Principle Award Recipient: JohnG Kenny
  • National Health and Medical Research Council (Award GNT1145631)
    • Principle Award Recipient: TimothyP Stinear
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-06-07
2025-06-13
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GROND: a quality-checked and publicly available database of full-length 16S-ITS-23S rRNA operon sequences
M Gen 10, 001255 (2024); https://doi.org/10.1099/mgen.0.001255
/content/journal/mgen/10.1099/mgen.0.001255
/content/journal/mgen/10.1099/mgen.0.001255
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