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Microbial Genomics logo Microbial Genomics

Volume 10, Issue 1

Phenotypic and genotypic characterization of sp. nov. and sp. nov., isolated from marine sediment, and genomic properties of the genus Open Access

Abstract

In this study, two novel bacterial strains were isolated from coastal sediment of Weihai, China. The two strains were Gram-stain-negative and facultatively aerobic, designated 3-1745 and A346. Based on phenotypic, genetic and phylogenetic properties, strains 3-1745 and A346 represent two novel species of the genus . The results of genome analysis revealed many central carbohydrate metabolism pathways such as gluconeogenesis, pyruvate oxidation, tricyclic acid cycle, pentose phosphate pathway and PRPP biosynthesis in the genus . The ability of strains 3-1745 and A346 to utilize volatile fatty acids was experimentally confirmed. Polyhydroxyalkanoate synthases (PhaA, PhaB and PhaC) for the synthesis of polyhydroxyalkanoates were prevalent in the genus . Multiple BGCs (biosynthetic gene clusters) including betalactone, ectoine, ranthipeptide, redox-cofactor, RiPPs (ribosomally synthesized post-translationally modified peptides) and T3PKS (polyketide synthases) in the genome of the genus were found. Additional genome analyses suggested that the genus contained diverse potential mechanisms of salt tolerance and mainly utilized oligosaccharides. This is the first report on broad genomic analyses of the genus with the description of two novel species and potential ecological and biotechnological implications.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): comparative genomic analysis , Marinobacterium , polyhydroxyalkanoates and polyphasic taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Shandong Province (Award ZR2022QC106)
    • Principal Award Recipient: Meng-QiYe
  • National Natural Science Foundation of China (Award 32200003)
    • Principal Award Recipient: Meng-QiYe
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2022A1515110773)
    • Principal Award Recipient: Meng-QiYe
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2024-01-24
2025-12-06

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/content/journal/mgen/10.1099/mgen.0.001182
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Phenotypic and genotypic characterization of Marinobacterium weihaiense sp. nov. and Marinobacterium marinum sp. nov., isolated from marine sediment, and genomic properties of the genus Marinobacterium
M Gen 10, 001182 (2024); https://doi.org/10.1099/mgen.0.001182
/content/journal/mgen/10.1099/mgen.0.001182
/content/journal/mgen/10.1099/mgen.0.001182
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