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Volume 10, Issue 1

Complete genomes of type B(F) isolates associated with a 1995 foodborne botulism outbreak from commercial pâté reveals a recombination event disrupting the gene Open Access

Abstract

Foodborne botulism is a neuroparalytic disease caused by ingestion of foods contaminated with botulinum neurotoxin (BoNT), produced by . In 1995 a husband and wife from Québec, Canada, were hospitalized for several months with prolonged muscle paralysis after ingesting a commercial . Examination of faecal samples from both patients and the pâté produced viable Group I (proteolytic) type B from each of the three samples. Whole genome sequencing revealed that all three isolates contain identical and genes encoded on a plasmid. Both faecal isolate genomes were identical in chromosome and plasmid length, as well as gene content. The genome of the pâté isolate was nearly identical to that of the faecal isolates with the notable difference of a missing 13-gene insertion on the cluster disrupting the gene. Examination of the insertion revealed several mobile genetic elements that participate in recombination.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): botulinum neurotoxin (BoNT) , botulism , Clostridium botulinum , foodborne botulism , outbreak and recombination
© 2023 Crown Copyright
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-01-04
2025-02-08
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/content/journal/mgen/10.1099/mgen.0.001169
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Complete genomes of Clostridium botulinum type B(F) isolates associated with a 1995 foodborne botulism outbreak from commercial pâté reveals a recombination event disrupting the ntnh gene
M Gen 10, 001169 (2024); https://doi.org/10.1099/mgen.0.001169
/content/journal/mgen/10.1099/mgen.0.001169
/content/journal/mgen/10.1099/mgen.0.001169
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