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Volume 9, Issue 10

Comparative genomics of , a fast-growing pathogen of wild Open Access

Abstract

is a fast-growing species isolated from wild and first described in 2013. isolates have been associated with arthritis, kerato conjunctivitis, pneumonia and septicemia, but were also recovered from apparently healthy animals. To better understand what defines this species, we performed a genomic survey on 14 strains collected from free-ranging or zoo-housed animals between 1987 and 2017, mostly in Europe. The average chromosome size of the strains was 1,040±0,024 kbp, with 24 % G+C and 852±31 CDS. The core genome and pan-genome of the species contained 628 and 1312 protein families, respectively. The strains displayed a relatively closed pan-genome, with many features and putative virulence factors shared with species from the cluster, including the MIB-MIP Ig cleavage system, a repertoire of DUF285 surface proteins and a complete biosynthetic pathway for galactan. genomes were found to be mostly syntenic, although repertoires of mobile genetic elements, including Mycoplasma Integrative and Conjugative Elements, insertion sequences, and a single plasmid varied. Phylogenetic- and gene content analyses confirmed that was closer to the cluster than to the ruminant species and . Ancestral genome reconstruction showed that the emergence of the species was associated with the gain of 17 gene families, some of which encode defence enzymes and surface proteins, and the loss of 25 others, some of which are involved in sugar transport and metabolism. This comparative study suggests that the cluster could be extended to include . We also find evidence that the specific organization and structure of the DnaA boxes around the of may contribute to drive the remarkable fast growth of this minimal bacterium.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): M. mycoides cluster , mobile elements , mollicutes , Mycoplasma feriruminatoris , oriC , polysaccharide biosynthesis and ruminant pathogen
Funding
This study was supported by the:
  • International Development Research Centre (Award 108625)
    • Principle Award Recipient: PascalSIRAND-PUGNET
  • International Development Research Centre (Award 108625)
    • Principle Award Recipient: CaroleLartigue
  • International Development Research Centre (Award 108625)
    • Principle Award Recipient: VincentBaby
  • International Development Research Centre (Award 108625)
    • Principle Award Recipient: JoergJores
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Comparative genomics of Mycoplasma feriruminatoris, a fast-growing pathogen of wild Caprinae
M Gen 9, 001112 (2023); https://doi.org/10.1099/mgen.0.001112
/content/journal/mgen/10.1099/mgen.0.001112
/content/journal/mgen/10.1099/mgen.0.001112
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