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Microbial Genomics logo Microbial Genomics

Volume 9, Issue 9

One size does not fit all – Trehalose metabolism by is variable across the five phylogenetic lineages Open Access

Abstract

, the leading cause of antibiotic-associated diarrhoea worldwide, is a genetically diverse species which can metabolise a number of nutrient sources upon colonising a dysbiotic gut environment. Trehalose, a disaccharide sugar consisting of two glucose molecules bonded by an α 1,1-glycosidic bond, has been hypothesised to be involved in the emergence of hypervirulence due to its increased utilisation by the RT027 and RT078 strains. Here, growth in trehalose as the sole carbon source was shown to be non-uniform across representative strains, even though the genes for its metabolism were induced. Growth in trehalose reduced the expression of genes associated with toxin production and sporulation in the R20291 (RT027) and M120 (RT078) strains , suggesting an inhibitory effect on virulence factors. Interestingly, the R20291 TreR transcriptional regulatory protein appeared to possess an activator function as its DNA-binding ability was increased in the presence of its effector, trehalose-6-phosphate. Using RNA-sequencing analysis, we report the identification of a putative trehalose metabolism pathway which is induced during growth in trehalose: this has not been previously described within the species. These data demonstrate the metabolic diversity exhibited by which warrants further investigation to elucidate the molecular basis of trehalose metabolism within this important gut pathogen.

  • Received:
  • Accepted:
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Keyword(s): carbohydrates , CDI , Clostridioides difficile , metabolism , nutrients , phylogenetic clades and trehalose
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-09-28
2025-01-16
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One size does not fit all – Trehalose metabolism by Clostridioides difficile is variable across the five phylogenetic lineages
M Gen 9, 001110 (2023); https://doi.org/10.1099/mgen.0.001110
/content/journal/mgen/10.1099/mgen.0.001110
/content/journal/mgen/10.1099/mgen.0.001110
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