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Volume 9, Issue 7

High subtelomeric GC content in the genome of a zoonotic species Open Access

Abstract

is a zoonotic species causing cryptosporidiosis in humans in addition to its natural hosts dogs and other fur animals. To understand the genetic basis for host adaptation, we sequenced the genomes of from dogs, minks, and foxes and conducted a comparative genomics analysis. While the genomes of have similar gene contents and organisations, they (~41.0 %) and (39.6 %) have GC content much higher than other spp. (24.3–32.9 %) sequenced to date. The high GC content is mostly restricted to subtelomeric regions of the eight chromosomes. Most of these GC-balanced genes encode specific proteins that have intrinsically disordered regions and are involved in host-parasite interactions. Natural selection appears to play a more important role in the evolution of codon usage in GC-balanced , and most of the GC-balanced genes have undergone positive selection. While the identity in whole genome sequences between the mink- and dog-derived isolates is 99.9 % (9365 SNVs), it is only 96.0 % (362 894 SNVs) between them and the fox-derived isolate. In agreement with this, the fox-derived isolate possesses more subtelomeric genes encoding invasion-related protein families. Therefore, the change in subtelomeric GC content appears to be responsible for the more GC-balanced genomes, and the fox-derived isolate could represent a new species.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): codon usage , Cryptosporidium canis , GC content , host-adaptive evolution and Whole genome sequencing
Funding
This study was supported by the:
  • Innovation Team Project of Guangdong Universities (Award 2019KCXTD001)
    • Principle Award Recipient: YaoyuFeng
  • 111 Project of Guangdong Universities (Award D20008)
    • Principle Award Recipient: LihuaXiao
  • National Natural Science Foundation of China (Award 32150710530)
    • Principle Award Recipient: LihuaXiao
  • National Natural Science Foundation of China (Award 31820103014)
    • Principle Award Recipient: LihuaXiao
  • Guangdong Major Project of Basic and Applied Basic Research (Award 2020B0301030007)
    • Principle Award Recipient: LihuaXiao
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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High subtelomeric GC content in the genome of a zoonotic Cryptosporidium species
M Gen 9, 001052 (2023); https://doi.org/10.1099/mgen.0.001052
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