1887

Abstract

The type VIIb protein secretion system (T7SSb) is found in (firmicute) bacteria and has been shown to mediate interbacterial competition. EssC is a membrane-bound ATPase that is a critical component of the T7SSb and plays a key role in substrate recognition. Prior analysis of available genome sequences of the foodborne bacterial pathogen has shown that although the T7SSb was encoded as part of the core genome, EssC could be found as one of seven different sequence variants. While each sequence variant was associated with a specific suite of candidate substrate proteins encoded immediately downstream of , many LXG-domain proteins were encoded across multiple sequence variants. Here, we have extended this analysis using a diverse collection of 37 930 . genomes. We have identified a rare eighth variant of EssC present in ten lineage III genomes. These genomes also encode a large toxin of the rearrangement hotspot (Rhs) repeat family adjacent to , along with a probable immunity protein and three small accessory proteins. We have further identified nine novel LXG-domain proteins, and four additional chromosomal hotspots across genomes where LXG proteins can be encoded. The eight EssC variants were also found in other species, with additional novel EssC types also identified. Across the genus, species frequently encoded multiple EssC types, indicating that T7SSb diversity is a primary feature of the genus .

Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/M011186/1)
    • Principle Award Recipient: StephenR Garrett
  • Wellcome Trust (Award 224151/Z/21/Z)
    • Principle Award Recipient: TracyPalmer
  • Wellcome Trust (Award 10183/Z/15/Z)
    • Principle Award Recipient: TracyPalmer
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-06-06
2024-07-13
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