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Volume 9, Issue 5

Development of databases for O- and K-antigen genotyping Open Access

Abstract

is an important food-borne human pathogen and presents immunogenic surface polysaccharides, which can be used to distinguish problematic and disease-causing lineages. is divided in 16 O-serotypes (O-antigen) and 71 K-serotypes (K-antigen). Agglutination tests are still the gold standard for serotyping, but many isolates are not typable by agglutination. An alternative for agglutination tests is genotyping using whole-genome sequencing data, by which K- and O- genotypes have been curated and identified previously for other clinically relevant organisms with the software tool . In this study, isolates were serotyped and sequenced, and all known and several novel O- and K-loci were identified. We developed databases for all O- and K-loci after manual curation of the loci. In our study, we could genotype the O- and K-loci of 98 and 93 % of the genomes, respectively, with a confidence score higher than ‘none’. The newly developed databases with the identified O- and K-loci can be used to identify the O- and K-genotypes of isolates from genome sequences.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genotyping , K-locus , O-locus , serotyping , Vibrio parahaemolyticus and whole-genome sequencing
Funding
This study was supported by the:
  • 2018ZX10305409-003 (Award 2018ZX10305409-003)
    • Principle Award Recipient: HongyouChen
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-05-02
2024-10-12
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/content/journal/mgen/10.1099/mgen.0.001007
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Development of Kaptive databases for Vibrio parahaemolyticus O- and K-antigen genotyping
M Gen 9, 001007 (2023); https://doi.org/10.1099/mgen.0.001007
/content/journal/mgen/10.1099/mgen.0.001007
/content/journal/mgen/10.1099/mgen.0.001007
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