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Volume 8, Issue 12

Characterization of from rice planthoppers reveals a clade of rice-associated undergoing genome reduction Open Access

Abstract

is a bacterium that is found in many agronomic crops and agricultural pests. Here, we isolated a strain (Lstr) from the rice planthopper , a notorious pest that feeds on rice plant sap and transmits rice viruses, in order to examine its genome and biology. Lstr is an insect symbiont that is pathogenic to the host insect and appears to mostly inhabit the gut. Its pathogenicity thus raises the possibility of using the Lstr strain as a biological agent. To this end, we analysed the genome of the Lstr strain and compared it with the genomes of other species. Our analysis of these genomes shows that can be divided into two mono-phylogenetic clades (clades one and two). The Lstr strain belongs to clade two and is grouped with strains that were isolated from rice or rice-associated samples. A comparative genomic analysis shows that clade two differs from clade one in many genomic characteristics including genome structures, mobile elements, and categories of coding proteins. The genomes of clade two . are significantly smaller, have much fewer coding sequences but more pseudogenes than those of clade one, suggesting that clade two species are at the early stage of genome reduction. On the other hand, has a type VI secretion system that is highly variable but cannot be separated by clades. These results clarify our understanding of ’ phylogenetic diversity and provide clues to the interactions between , host insect, and plant that may lead to advances in rice protection and pest control.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): comparative genomics , Laodelphax striatellus , Pantoea ananatis , pathogen , rice and T6SS
Funding
This study was supported by the:
  • Natural Science Foundation of Jiangsu Province (Award BK20211213)
    • Principle Award Recipient: Xiao-LiBing
  • Central University Basic Research Fund of China (Award KJQN202110)
    • Principle Award Recipient: Xiao-LiBing
  • National Natural Science Foundation of China (Award 32020103011)
    • Principle Award Recipient: Xiao-YueHong
  • National Natural Science Foundation of China (Award 32001905)
    • Principle Award Recipient: Xiao-LiBing
  • National Key R&D Program of China (Award 2021YFD1401100)
    • Principle Award Recipient: BingXiao-Li
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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Characterization of Pantoea ananatis from rice planthoppers reveals a clade of rice-associated P. ananatis undergoing genome reduction
M Gen 8, 000907 (2022); https://doi.org/10.1099/mgen.0.000907
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