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Volume 8, Issue 6

Genome plasticity driven by aneuploidy and loss of heterozygosity in Open Access

Abstract

the causative agent of Chagas disease shows a marked genetic diversity and divided into at least six Discrete Typing Units (DTUs). High intra genetic variability has been observed in the TcI DTU, the most widely distributed DTU, where patterns of genomic diversity can provide information on ecological and evolutionary processes driving parasite population structure and genome organization. Chromosomal aneuploidies and rearrangements across multigene families represent an evidence of genome plasticity. We explored genomic diversity among 18 Colombian I clones and 15 . I South American strains. Our results confirm high genomic variability, heterozygosity and presence of a clade compatible with the TcI genotype, described for strains from humans in Colombia and Venezuela. TcI showed high structural plasticity across the geographical region studied. Differential events of whole and segmental aneuploidy (SA) along chromosomes even between clones from the same strain were found and corroborated by the depth and allelic frequency. We detected loss of heterozygosity (LOH) events in different chromosomes, however, the size and location of segments under LOH varied between clones. Genes adjacent to breakpoints were evaluated, and retrotransposon hot spot genes flanked the beginning of segmental aneuploidies. Our results suggest that genomes, like those of , may have a highly unstable structure and there is now an urgent need to design experiments to explore any potential adaptive role for the plasticity observed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aneuploidy , genome , loss of heterozygosity , segmental allele frequency , TcI and Trypanosoma cruzi
Funding
This study was supported by the:
  • Pew Charitable Trusts (Award Latinamerican fellow)
    • Principle Award Recipient: JUANDAVID RAMIREZ
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.

Erratum

An erratum has been published for this content:
Corrigendum: Genome plasticity driven by aneuploidy and loss of heterozygosity in
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2022-06-24
2024-04-16
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Genome plasticity driven by aneuploidy and loss of heterozygosity in Trypanosoma cruzi
M Gen 8, 000843 (2022); https://doi.org/10.1099/mgen.0.000843
/content/journal/mgen/10.1099/mgen.0.000843
/content/journal/mgen/10.1099/mgen.0.000843
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