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Volume 8, Issue 5

Genomic epidemiology and temperature dependency of hypermucoviscous in Japan Open Access

Abstract

(Kp) has emerged as a global life-threatening pathogen owing to its multidrug resistance and hypervirulence phenotype. Several fatal outbreaks of carbapenem-resistant hypervirulent Kp have been reported recently. Hypermucoviscosity (HMV) is a phenotype commonly associated with hypervirulence of Kp, which is usually regulated by or (regulators of the mucoid phenotype). Here, we found that temperature was important in the HMV phenotype of Kp, and the impact of temperature on HMV was not uniform among strains. We investigated the HMV phenotype at 37 °C and room temperature (20–25 °C) in 170 clinically isolated hypermucoviscous Kp strains in Japan and analysed the association between the HMV phenotype, virulence genes and antimicrobial resistance (AMR) genes. String length distribution at different temperatures was correlated with the genomic population of Kp. The strains carrying / frequently showed the HMV phenotype at 37 °C, while the strains negative for these genes tended to show the HMV phenotype at room temperature. Hypervirulent Kp clusters carrying / without extended-spectrum beta-lactamases (ESBL)/carbapenemases produced higher string lengths at 37 °C than at room temperature, and were mostly isolated from the respiratory tract. Other HMV strains showed distinct characteristics of not carrying / but were positive for ESBL/carbapenemases, with a higher string length at room temperature than at 37 °C, and were frequently isolated from bloodstream infections. In total, 21 (13.5 %) HMV isolates carried ESBL and carbapenemases, among which five isolates were carbapenem-resistant hypervirulent Kp with a pLVPK-like plasmid (an epidemic virulence plasmid) and a pKPI-6-like plasmid (an epidemic -bearing plasmid in Japan), suggesting the convergence of worldwide hypervirulence and epidemic AMR in Japan.

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Keyword(s): hypermucoviscous , hypervirulent , Klebsiella pneumoniae , multidrug resistance and string test
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award JP18K07112)
    • Principle Award Recipient: ShizuoKayama
  • Japan Society for the Promotion of Science (Award JP21K16947)
    • Principle Award Recipient: MiNguyen-Tra Le
  • Japan Agency for Medical Research and Development (Award 21fk0108604j0001)
    • Principle Award Recipient: MotoyukiSugai
  • Japan Agency for Medical Research and Development (Award 20fk0108132j0001)
    • Principle Award Recipient: MotoyukiSugai
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-05-27
2024-04-25
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Genomic epidemiology and temperature dependency of hypermucoviscous Klebsiella pneumoniae in Japan
M Gen 8, 000827 (2022); https://doi.org/10.1099/mgen.0.000827
/content/journal/mgen/10.1099/mgen.0.000827
/content/journal/mgen/10.1099/mgen.0.000827
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