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Volume 8, Issue 2

Reciprocally rewiring and repositioning the Integration Host Factor (IHF) subunit genes in serovar Typhimurium: impacts on physiology and virulence Open Access

Abstract

The Integration Host Factor (IHF) is a heterodimeric nucleoid-associated protein that plays roles in bacterial nucleoid architecture and genome-wide gene regulation. The and genes encode the subunits and are located 350 kbp apart, in the Right replichore of the chromosome. IHF is composed of one IhfA and one IhfB subunit. Despite this 1 : 1 stoichiometry, MS revealed that IhfB is produced in 2-fold excess over IhfA. We re-engineered to exchange reciprocally the protein-coding regions of and such that each relocated protein-encoding region was driven by the expression signals of the other’s gene. MS showed that in this 'rewired' strain, IhfA is produced in excess over IhfB, correlating with enhanced stability of the hybrid mRNA that was expressed from the promoter. Nevertheless, the rewired strain grew at a similar rate to the wild-type and was similar in competitive fitness. However, compared to the wild-type, it was less motile, had growth-phase-specific reductions in SPI-1 and SPI-2 gene expression, and was engulfed at a higher rate by RAW macrophage. Our data show that while exchanging the physical locations of its genes and the rewiring of their regulatory circuitry are well tolerated in , genes involved in the production of type 3 secretion systems exhibit dysregulation accompanied by altered phenotypes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): gene regulation , genome organization , genome rewiring , Integration Host Factor (IHF) , proteome and Salmonella enterica serovar Typhimurium
Funding
This study was supported by the:
  • Wellcome Trust (Award 206194)
    • Principle Award Recipient: NicholasR Thomson
  • Science Foundation Ireland (Award 13/IA/1875)
    • Principle Award Recipient: CharlesJ. Dorman
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-04-25
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http://instance.metastore.ingenta.com/content/journal/mgen/10.1099/mgen.0.000768
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Reciprocally rewiring and repositioning the Integration Host Factor (IHF) subunit genes in Salmonella enterica serovar Typhimurium: impacts on physiology and virulence
M Gen 8, 000768 (2022); https://doi.org/10.1099/mgen.0.000768
/content/journal/mgen/10.1099/mgen.0.000768
/content/journal/mgen/10.1099/mgen.0.000768
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