@article{mbs:/content/journal/mgen/10.1099/mgen.0.000746, author = "Ndlangisa, Kedibone M. and du Plessis, Mignon and Lo, Stephanie and de Gouveia, Linda and Chaguza, Chrispin and Antonio, Martin and Kwambana-Adams, Brenda and Cornick, Jennifer and Everett, Dean B. and Dagan, Ron and Hawkins, Paulina A. and Beall, Bernard and Corso, Alejandra and Grassi Almeida, Samanta Cristine and Ochoa, Theresa J. and Obaro, Stephen and Shakoor, Sadia and Donkor, Eric S. and Gladstone, Rebecca A. and Ho, Pak Leung and Paragi, Metka and Doiphode, Sanjay and Srifuengfung, Somporn and Ford, Rebecca and Moïsi, Jennifer and Saha, Samir K. and Bigogo, Godfrey and Sigauque, Betuel and Eser, Özgen Köseoglu and Elmdaghri, Naima and Titov, Leonid and Turner, Paul and Kumar, K. L. Ravi and Kandasamy, Rama and Egorova, Ekaterina and IP, Margaret and Breiman, Robert F. and Klugman, Keith P. and McGee, Lesley and Bentley, Stephen D. and von Gottberg, Anne and The Global Pneumococcal Sequencing Consortium", title = "A Streptococcus pneumoniae lineage usually associated with pneumococcal conjugate vaccine (PCV) serotypes is the most common cause of serotype 35B invasive disease in South Africa, following routine use of PCV", journal= "Microbial Genomics", year = "2022", volume = "8", number = "4", pages = "", doi = "https://doi.org/10.1099/mgen.0.000746", url = "https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000746", publisher = "Microbiology Society", issn = "2057-5858", type = "Journal Article", keywords = "serotype 35B", keywords = "South Africa", keywords = "global pneumococcal sequence cluster", keywords = "Streptococcus pneumoniae", eid = "000746", abstract = "Pneumococcal serotype 35B is an important non-conjugate vaccine (non-PCV) serotype. Its continued emergence, post-PCV7 in the USA, was associated with expansion of a pre-existing 35B clone (clonal complex [CC] 558) along with post-PCV13 emergence of a non-35B clone previously associated with PCV serotypes (CC156). This study describes lineages circulating among 35B isolates in South Africa before and after PCV introduction. We also compared 35B isolates belonging to a predominant 35B lineage in South Africa (GPSC5), with isolates belonging to the same lineage in other parts of the world. Serotype 35B isolates that caused invasive pneumococcal disease in South Africa in 2005–2014 were characterized by whole-genome sequencing (WGS). Multi-locus sequence types and global pneumococcal sequence clusters (GPSCs) were derived from WGS data of 63 35B isolates obtained in 2005–2014. A total of 262 isolates that belong to GPSC5 (115 isolates from South Africa and 147 from other countries) that were sequenced as part of the global pneumococcal sequencing (GPS) project were included for comparison. Serotype 35B isolates from South Africa were differentiated into seven GPSCs and GPSC5 was most common (49 %, 31/63). While 35B was the most common serotype among GPSC5/CC172 isolates in South Africa during the PCV13 period (66 %, 29/44), 23F was the most common serotype during both the pre-PCV (80 %, 37/46) and PCV7 period (32 %, 8/25). Serotype 35B represented 15 % (40/262) of GPSC5 isolates within the global GPS database and 75 % (31/40) were from South Africa. The predominance of the GPSC5 lineage within non-vaccine serotype 35B, is possibly unique to South Africa and warrants further molecular surveillance of pneumococci.", }