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Volume 7, Issue 11

Bilateral symmetry of linear streptomycete chromosomes Open Access

Abstract

Here, we characterize an uncommon set of telomeres from ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes of replicon ends found amongst streptomycetes. Closed replicons of streptomycete chromosomes were organized with respect to their phylogeny and physical orientation, which demonstrated that different telomeres were not associated with particular clades and are likely shared amongst different strains by plasmid-driven horizontal gene transfer. Furthermore, we identified a ~50 kb origin island with conserved synteny that is located at the core of all streptomycete chromosomes and forms an axis around which symmetrical chromosome inversions can take place. Despite this chromosomal bilateral symmetry, a bias in sites to the right of is maintained across the family and suggests that the formation of ParB/ nucleoprotein complexes on the right replichore is a conserved feature in streptomycetes. Consequently, our studies reveal novel features of linear bacterial replicons that, through their manipulation, may lead to improvements in growth and productivity of this important industrial group of bacteria.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bilateral symmetry , linear chromosome , oriC and Streptomyces
Funding
This study was supported by the:
  • University of Strathclyde
    • Principle Award Recipient: R MarkDavid
  • IBioIC
    • Principle Award Recipient: R HerronPaul
  • Biotechnology and Biological Sciences Research Council (Award BB/M018792/1)
    • Principle Award Recipient: R HerronPaul
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-11-15
2024-04-23
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Bilateral symmetry of linear streptomycete chromosomes
M Gen 7, 000692 (2021); https://doi.org/10.1099/mgen.0.000692
/content/journal/mgen/10.1099/mgen.0.000692
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