1887

Abstract

We report here the complete genome sequence of the widely studied serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

Funding
This study was supported by the:
  • Wellcome Trust (Award 098051)
    • Principle Award Recipient: MatthewT.G. Holden
  • National Natural Science Foundation of China (Award 31520103917)
    • Principle Award Recipient: LianchengLei
  • Biotechnology and Biological Sciences Research Council (Award BB/S005897/1)
    • Principle Award Recipient: PaulR. Langford
  • Biotechnology and Biological Sciences Research Council (Award BB/S002103/1)
    • Principle Award Recipient: PaulR. Langford
  • Biotechnology and Biological Sciences Research Council (Award BB/G020744/1)
    • Principle Award Recipient: AndrewN. Rycroft
  • Biotechnology and Biological Sciences Research Council (Award BB/G018553/1)
    • Principle Award Recipient: PaulR. Langford
  • Biotechnology and Biological Sciences Research Council (Award BB/G019274/1)
    • Principle Award Recipient: DuncanJ. Maskell
  • Biotechnology and Biological Sciences Research Council (Award BB/G019177/1)
    • Principle Award Recipient: BrendanW. Wren
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-11-24
2024-04-25
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