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Volume 7, Issue 11

Whole set of constitutive promoters for RpoN sigma factor and the regulatory role of its enhancer protein NtrC in K-12 Open Access

Abstract

The promoter selectivity of RNA polymerase (RNAP) is determined by its promoter-recognition sigma subunit. The model prokaryote K-12 contains seven species of the sigma subunit, each recognizing a specific set of promoters. Using genomic SELEX (gSELEX) screening , we identified the whole set of ‘constitutive’ promoters recognized by the reconstituted RNAP holoenzyme alone, containing RpoD (σ), RpoS (σ), RpoH (σ), RpoF (σ) or RpoE (σ), in the absence of other supporting regulatory factors. In contrast, RpoN sigma (σ), involved in expression of nitrogen-related genes and also other cellular functions, requires an enhancer (or activator) protein, such as NtrC, for transcription initiation. In this study, a series of gSELEX screenings were performed to search for promoters recognized by the RpoN RNAP holoenzyme in the presence and absence of the major nitrogen response enhancer NtrC, the best-characterized enhancer. Based on the RpoN holoenzyme-binding sites, a total of 44 to 61 putative promoters were identified, which were recognized by the RpoN holoenzyme alone. In the presence of the enhancer NtrC, the recognition target increased to 61–81 promoters. Consensus sequences of promoters recognized by RpoN holoenzyme in the absence and presence of NtrC were determined. The promoter activity of a set of NtrC-dependent and -independent RpoN promoters was verified under nitrogen starvation, in the presence and absence of RpoN and/or NtrC. The promoter activity of some RpoN-recognized promoters increased in the absence of RpoN or NtrC, supporting the concept that the promoter-bound NtrC-enhanced RpoN holoenzyme functions as a repressor against RpoD holoenzyme. Based on our findings, we propose a model in which the RpoN holoenzyme fulfils the dual role of repressor and transcriptase for the same set of genes. We also propose that the promoter recognized by RpoN holoenzyme in the absence of enhancers is the ‘repressive’ promoter. The presence of high-level RpoN sigma in growing K-12 in rich medium may be related to the repression role of a set of genes needed for the utilization of ammonia as a nitrogen source in poor media. The list of newly identified regulatory targets of RpoN provides insight into survival under nitrogen-depleted conditions in nature.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Escherichia coli , gSELEX , nitrogen metabolism , NtrC , RNA polymerase and RpoN sigma factor
Funding
This study was supported by the:
  • japan society for the promotion of science (Award 25430173)
    • Principle Award Recipient: AkiraIshihama
  • japan society for the promotion of science (Award 18310133)
    • Principle Award Recipient: AkiraIshihama
  • japan society for the promotion of science (Award 19K06618)
    • Principle Award Recipient: TomohiroShimada
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-11-17
2024-04-18
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Whole set of constitutive promoters for RpoN sigma factor and the regulatory role of its enhancer protein NtrC in Escherichia coli K-12
M Gen 7, 000653 (2021); https://doi.org/10.1099/mgen.0.000653
/content/journal/mgen/10.1099/mgen.0.000653
/content/journal/mgen/10.1099/mgen.0.000653
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