Whole genome sequencing reveals great diversity of spp in prawns at retail Open Access

Abstract

Consumption of prawns as a protein source has been on the rise worldwide with seafood identified as the predominant attributable source of human vibriosis. However, surveillance of non-cholera is limited both in public health and in food. Using a population- and market share-weighted study design, 211 prawn samples were collected and cultured for spp. Contamination was detected in 46 % of samples, and multiple diverse isolates were obtained from 34 % of positive samples. Whole genome sequencing (WGS) and phylogenetic analysis illustrated a comprehensive view of species diversity in prawns available at retail, with no known pathogenicity markers identified in and . Antimicrobial resistance genes were found in 77 % of isolates, and 12 % carried genes conferring resistance to three or more drug classes. Resistance genes were found predominantly in , though multiple resistance genes were also identified in and . This study highlights the large diversity in derived from prawns at retail, even within a single sample. Although there was little evidence in this study that prawns are a major source of vibriosis in the UK, surveillance of non-cholera is very limited. This study illustrates the value of expanding WGS surveillance efforts of non-cholera Vibrios in the food chain to identify critical control points for food safety through the production system and to determine the full extent of the public health impact.

Funding
This study was supported by the:
  • food standards agency (Award FS101185)
    • Principle Award Recipient: AlisonMather
  • biotechnology and biological sciences research council (Award BBS/E/F/000PR10348)
    • Principle Award Recipient: AlisonMather
  • biotechnology and biological sciences research council (Award BB/R012504/1)
    • Principle Award Recipient: AlisonMather
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2021-09-29
2024-03-29
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