1887

Abstract

Enterotoxigenic (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

Funding
This study was supported by the:
  • BBSRC Institute Strategic Programme Microbes in the Food Chain (Award BB/R012504/1 and BBS/E/F/000PR10348)
    • Principle Award Recipient: RobertA. Kingsley
  • The Swedish Research Council
    • Principle Award Recipient: ÅsaSjoling
  • NIMR Cambridge BRC Antibiotic Resistance theme (Award ESPRC Vaccine-Hub grant)
    • Principle Award Recipient: GordonDougan
  • Marie Bashir Institute and Sydney Medical School Foundation (AU)
    • Principle Award Recipient: MoatazAbd El Ghany
  • Wellcome Trust
    • Principle Award Recipient: GordonDougan
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-06-10
2022-01-24
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