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Volume 7, Issue 5

Phenotypic and genomic characterization of a strain causing disease in provides insights into its niche adaptation and pathogenic mechanism Open Access

Abstract

The virulence of is variable depending on its virulence determinants. A strain, in which the virulence is governed by the and genes, can cause acute hepatopancreatic necrosis disease (AHPND) in shrimps. Some that are non-AHPND strains also cause shrimp diseases and result in huge economic losses, while their pathogenicity and pathogenesis remain unclear. In this study, a non-AHPND , TJA114, was isolated from diseased associated with a high mortality. To understand its virulence and adaptation to the external environment, whole-genome sequencing of this isolate was conducted, and its phenotypic profiles including pathogenicity, growth characteristics and nutritional requirements were investigated. Shrimps following artificial infection with this isolate presented similar clinical symptoms to the naturally diseased ones and generated obvious pathological lesions. The growth characteristics indicated that the isolate TJA114 could grow well under different salinity (10–55 p.p.t.), temperature (23–37 °C) and pH (6–10) conditions. Phenotype MicroArray results showed that this isolate could utilize a variety of carbon sources, amino acids and a range of substrates to help itself adapt to the high hyperosmotic and alkaline environments. Antimicrobial-susceptibility test showed that it was a multidrug-resistant bacterium. The whole-genomic analysis showed that this possessed many important functional genes associated with multidrug resistance, stress response, adhesions, haemolysis, putative secreted proteases, dedicated protein secretion systems and a variety of nutritional metabolic mechanisms. These annotated functional genes were confirmed by the phenotypic profiles. The results in this study indicated that this isolate possesses a high pathogenicity and strong environmental adaptability.

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  • Accepted:
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Keyword(s): niche adaptation , pathogenicity , Penaeus vannamei , Vibrio parahaemolyticus and whole-genome sequencing
Funding
This study was supported by the:
  • the Scientific Research and Innovation Project of Tianjin Agricultural University in 2019 (Award 2019XY038)
    • Principle Award Recipient: XueZhang
  • the Science and Technology Project in the Field of Social Development in Tianjin Binhai New Area (Award BHXQKJXM-SF-2018-34)
    • Principle Award Recipient: YadongHuang
  • the Science and Technology Innovation Project of Shandong province (Award 2018SDKJ0406-4)
    • Principle Award Recipient: HongliQi
  • The Scientific Programs of Tianjin City (Award 19JCTPJC60100)
    • Principle Award Recipient: LimeiChen
  • the Innovation Team of Tianjin Fisheries Research System (Award ITTFRS2017009)
    • Principle Award Recipient: SunJingfeng
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2021-05-05
2024-04-25
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Phenotypic and genomic characterization of a Vibrio parahaemolyticus strain causing disease in Penaeus vannamei provides insights into its niche adaptation and pathogenic mechanism
M Gen 7, 000549 (2021); https://doi.org/10.1099/mgen.0.000549
/content/journal/mgen/10.1099/mgen.0.000549
/content/journal/mgen/10.1099/mgen.0.000549
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