1887

Abstract

is a bacterium with a broad ecology spanning disease in humans, animals and plants, but also encompassing multiple beneficial interactions. It is a plant pathogen, a toxin-producing food-poisoning agent, and causes lung infections in people with cystic fibrosis (CF). Contrasting beneficial traits include antifungal production exploited by insects to protect their eggs, plant protective abilities and antibiotic biosynthesis. We explored the genomic diversity and specialized metabolic potential of 206 strains, phylogenomically defining 5 clades. Historical disease pathovars (pv.) pv. and pv. were distinct, while pv. and pv. were indistinguishable; soft-rot disease and CF infection were conserved across all pathovars. Biosynthetic gene clusters (BGCs) for toxoflavin, caryoynencin and enacyloxin were dispersed across , but bongkrekic acid and gladiolin production were clade-specific. Strikingly, 13 % of CF infection strains characterized were bongkrekic acid-positive, uniquely linking this food-poisoning toxin to this aspect of disease. Mapping the population biology and metabolite production of has shed light on its diverse ecology, and by demonstrating that the antibiotic trimethoprim suppresses bongkrekic acid production, a potential therapeutic strategy to minimize poisoning risk in CF has been identified.

Funding
This study was supported by the:
  • MatthewJenner , Biotechnology and Biological Sciences Research Council , (Award BB/R012121/1)
  • GregoryL. Challis , Biotechnology and Biological Sciences Research Council , (Award BB/M017982/1)
  • ThomasR. Connor , Medical Research Council , (Award MR/L015080/1)
  • GregoryL. Challis , Biotechnology and Biological Sciences Research Council , (Award BB/L023342/1)
  • EshwarMahenthiralingam , Biotechnology and Biological Sciences Research Council , (Award BB/L021692/1)
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/content/journal/mgen/10.1099/mgen.0.000515
2021-01-18
2021-02-26
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