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Volume 7, Issue 1

Genomic diversity of isolates from backyard chickens and guinea fowl in the Gambia Open Access

Abstract

Chickens and guinea fowl are commonly reared in Gambian homes as affordable sources of protein. Using standard microbiological techniques, we obtained 68 caecal isolates of from 10 chickens and 9 guinea fowl in rural Gambia. After Illumina whole-genome sequencing, 28 sequence types were detected in the isolates (4 of them novel), of which ST155 was the most common (22/68, 32 %). These strains span four of the eight main phylogroups of with phylogroups B1 and A being most prevalent. Nearly a third of the isolates harboured at least one antimicrobial resistance gene, while most of the ST155 isolates (14/22, 64 %) encoded resistance to ≥3 classes of clinically relevant antibiotics, as well as putative virulence factors, suggesting pathogenic potential in humans. Furthermore, hierarchical clustering revealed that several Gambian poultry strains were closely related to isolates from humans. Although the ST155 lineage is common in poultry from Africa and South America, the Gambian ST155 isolates belong to a unique cgMLST cluster comprising closely related (38–39 alleles differences) isolates from poultry and livestock from sub-Saharan Africa – suggesting that strains can be exchanged between poultry and livestock in this setting. Continued surveillance of and other potential pathogens in rural backyard poultry from sub-Saharan Africa is warranted.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): backyard poultry , chickens , Escherichia coli , genomic diversity , guinea fowl and ST155
Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/ CCG1860/1)
    • Principle Award Recipient: Nabil-FareedAlikhan
  • Biotechnology and Biological Sciences Research Council (Award BB/R012504/1)
    • Principle Award Recipient: MarkJohn PALLEN
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2020-11-30
2024-04-19
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Genomic diversity of Escherichia coli isolates from backyard chickens and guinea fowl in the Gambia
M Gen 7, 000484 (2021); https://doi.org/10.1099/mgen.0.000484
/content/journal/mgen/10.1099/mgen.0.000484
/content/journal/mgen/10.1099/mgen.0.000484
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