1887

Abstract

Mobile genetic elements (MGEs) are agents of bacterial evolution and adaptation. Genome sequencing provides an unbiased approach that has revealed an abundance of MGEs in prokaryotes, mainly plasmids and integrative conjugative elements. Nevertheless, many mobilomes, particularly those from environmental bacteria, remain underexplored despite their representing a reservoir of genes that can later emerge in the clinic. Here, we explored the mobilome of the family, focusing on strains from Brazilian Atlantic Forest soil. Novel and strains were identified, with the former ones harbouring linear and circular plasmids encoding the specialized type-VII secretion system (T7SS) and mobility-associated genes. In addition, we also identified a T4SS-mediated integrative conjugative element (ICEMyc226) encoding two T7SSs and a number of xenobiotic degrading genes. Our study uncovers the diversity of the mobilome, providing the evidence of an ICE in this bacterial family. Moreover, the presence of T7SS genes in an ICE, as well as plasmids, highlights the role of these mobile genetic elements in the dispersion of T7SS.

Funding
This study was supported by the:
  • Sergio Mascarenhas Morgado , Fundação Oswaldo Cruz
  • Sergio Mascarenhas Morgado , Coordenação de Aperfeiçoamento de Pessoal de Nível Superior , (Award 001)
  • Not Applicable , Conselho Nacional de Desenvolvimento Científico e Tecnológico
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2020-06-04
2020-08-09
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