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Volume 6, Issue 7

Genomics of Atlantic Forest strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS Open Access

Abstract

Mobile genetic elements (MGEs) are agents of bacterial evolution and adaptation. Genome sequencing provides an unbiased approach that has revealed an abundance of MGEs in prokaryotes, mainly plasmids and integrative conjugative elements. Nevertheless, many mobilomes, particularly those from environmental bacteria, remain underexplored despite their representing a reservoir of genes that can later emerge in the clinic. Here, we explored the mobilome of the family, focusing on strains from Brazilian Atlantic Forest soil. Novel and strains were identified, with the former ones harbouring linear and circular plasmids encoding the specialized type-VII secretion system (T7SS) and mobility-associated genes. In addition, we also identified a T4SS-mediated integrative conjugative element (ICEMyc226) encoding two T7SSs and a number of xenobiotic degrading genes. Our study uncovers the diversity of the mobilome, providing the evidence of an ICE in this bacterial family. Moreover, the presence of T7SS genes in an ICE, as well as plasmids, highlights the role of these mobile genetic elements in the dispersion of T7SS.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ICE , Mycobacteriaceae , Mycobacterium , plasmid , T4SS and T7SS
Funding
This study was supported by the:
  • Fundação Oswaldo Cruz
    • Principle Award Recipient: Sergio Mascarenhas Morgado
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award 001)
    • Principle Award Recipient: Sergio Mascarenhas Morgado
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-06-04
2024-04-18
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Genomics of Atlantic Forest Mycobacteriaceae strains unravels a mobilome diversity with a novel integrative conjugative element and plasmids harbouring T7SS
M Gen 6, e000382 (2020); https://doi.org/10.1099/mgen.0.000382
/content/journal/mgen/10.1099/mgen.0.000382
/content/journal/mgen/10.1099/mgen.0.000382
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