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Volume 6, Issue 6

Microevolution in the major outer membrane protein OmpA of Open Access

Abstract

is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formation, regulation of OM stability, permeability and antibiotic resistance. OmpA members are two‐domain proteins with an N‐terminal eight‐stranded β‐barrel domain with four external loops (ELs) interacting with the environment, and a C‐terminal periplasmic domain binding non‐covalently to the peptidoglycan. Here, we combined data from genome sequencing, phylogenetic and multilocus sequence analyses from 975 strains/isolates of the / complex (ACB), 946 from , to explore microevolutionary divergence. Five major variant groups were identified (V1 to V5) in , encompassing 52 different alleles coding for 23 different proteins. Polymorphisms were concentrated in five regions corresponding to the four ELs and the C‐terminal end, and provided evidence for intra‐genic recombination. variants were not randomly distributed across the . phylogeny, with the most frequent V1(lct)a1 allele found in most clonal complex 2 (CC2) strains and the second most frequent V2(lct)a1 allele in the majority of CC1 strains. Evidence was found for assortative exchanges of alleles not only between separate . lineages, but also different ACB species. The overall results have implications for evolution, epidemiology, virulence and vaccine design.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter baumannii , OmpA , outer membrane protein , protein evolution and recombination
Funding
This study was supported by the:
  • Agencia Nacional de Promoción Científica y Tecnológica (Award PICT-2015-1072)
    • Principle Award Recipient: Alejandro M. Viale
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2020-06-04
2024-04-20
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Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
M Gen 6, e000381 (2020); https://doi.org/10.1099/mgen.0.000381
/content/journal/mgen/10.1099/mgen.0.000381
/content/journal/mgen/10.1099/mgen.0.000381
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