1887

Abstract

To establish the prevalence of mobile colistin resistance () genes amongst isolates obtained through public health surveillance in England (April 2014 to September 2017), 33 205 . genome sequences obtained from human, food, animal and environmental isolates were screened for the presence of variants 1 to 8. The -positive genomes were assembled, annotated and characterized according to plasmid type. Nanopore sequencing was performed on six selected isolates with putative novel plasmids, and phylogenetic analysis was used to provide an evolutionary context for the most commonly isolated clones. Fifty-two -positive isolates were identified, of which 32 were positive for , 19 for and 1 for . The combination of Illumina and Nanopore sequencing identified three novel plasmids and one novel plasmid, as well as the presence of chromosomally integrated and . Monophasic serovar Typhimurium accounted for 27/52 (52 %) of the -positive isolates, with the majority clustering in clades associated with travel to Southeast Asia. Isolates in these clades were associated with a specific plasmid range and an additional extended-spectrum beta-lactamase genotype. Routine whole-genome sequencing for public health surveillance provides an effective screen for novel and emerging antimicrobial determinants, including . Complementary long-read technologies elucidated the genomic context of resistance determinants, offering insights into plasmid dissemination and linkage to other resistance genes.

Keyword(s): colistin , MCR , Salmonella and WGS
Funding
This study was supported by the:
  • Not Applicable , National Institute for Health Research
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2020-01-31
2020-02-28
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