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Volume 6, Issue 2

A survey of genes modulated by host cell infection Open Access

Abstract

Here, we report comprehensive transcriptomic profiles from under conditions that mimic the first stages of bacterial infection in a highly differentiated adenocarcinoma epithelial cell line. Our transcriptomic adenocarcinoma approach allows us to measure the expression dynamics and regulation of bacterial virulence and response factors in real time, and is a novel strategy for clarifying the role of infection in colorectal cancer (CRC) progression. Our data show that: (i) infection alters metabolic and functional pathways in , allowing the bacterium to adapt to the host-imposed milieu; (ii) infection also stimulates the expression of genes required to help induce and promote a hypoxic and inflammatory microenvironment in the host; and (iii) invasion occurs by a haematogenous route of infection. Our study identifies novel gene targets from that are activated during invasion and which may aid in determining how this species invades and promotes disease within the human gastrointestinal tract. These invasion-specific genes may be useful as biomarkers for CRC progression in a host and could also assist in the development of new diagnostic tools and treatments (such as vaccines or small molecule drug targets), which will be able to combat infection and inflammation in the host while circumventing the potential problem of tolerization.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Fusobacterium nucleatum , infection and transcriptome
Funding
This study was supported by the:
  • Canadian Cancer Society Research Institute (Award i2I17-1)
    • Principle Award Recipient: Robert A Holt
  • Canadian Cancer Society Research Institute (Award i2I17-1)
    • Principle Award Recipient: Emma Allen-Vercoe
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2019-10-29
2024-04-16
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A survey of Fusobacterium nucleatum genes modulated by host cell infection
M Gen 6, e000300 (2020); https://doi.org/10.1099/mgen.0.000300
/content/journal/mgen/10.1099/mgen.0.000300
/content/journal/mgen/10.1099/mgen.0.000300
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