1887

Abstract

doi: 10.1099/mgen.0.000121.001.

Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in children below 5 years of age in endemic areas, and is a primary cause of diarrhoea in travellers visiting developing countries. Epidemiological analysis of E. coli pathovars is traditionally carried out based on the results of serotyping. However, genomic analysis of a global ETEC collection of 362 isolates taken from patients revealed nine novel O-antigen biosynthesis gene clusters that were previously unrecognized, and have collectively been called unclassified. When put in the context of all isolates sequenced, one of the novel O-genotypes, OgN5, was found to be the second most common ETEC O-genotype causing disease, after O6, in a globally representative ETEC collection. It’s also clear that ETEC OgN5 isolates have spread globally. These novel O-genotypes have now been included in our comprehensive O-genotyping scheme, and can be detected using a PCR-based and an in silico typing method. This will assist in epidemiological studies, as well as in ETEC vaccine development.

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2017-07-03
2019-09-20
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References

  1. Kotloff KL, Nataro JP, Blackwelder WC, Nasrin D, Farag TH et al. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 2013;382:209–222 [CrossRef][PubMed]
    [Google Scholar]
  2. WHO Diarrhoeal diseases: enterotoxigenic Escherichia coli (ETEC)The Initiative for Vaccine Research. (Updated February 2009) Geneva: World Health Organization; 2009;www.who.int/vaccine_research/diseases/diarrhoeal/en/index4.html
    [Google Scholar]
  3. von Mentzer A, Connor TR, Wieler LH, Semmler T, Iguchi A et al. Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distribution. Nat Genet 2014;46:1321–1326 [CrossRef][PubMed]
    [Google Scholar]
  4. Iguchi A, Iyoda S, Kikuchi T, Ogura Y, Katsura K et al. A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster. DNA Res 2015;22:101–107 [CrossRef][PubMed]
    [Google Scholar]
  5. Liu B, Knirel YA, Feng L, Perepelov AV, Senchenkova SN et al. Structure and genetics of Shigella O antigens. FEMS Microbiol Rev 2008;32:627–653 [CrossRef][PubMed]
    [Google Scholar]
  6. DebRoy C, Fratamico PM, Yan X, Baranzoni G, Liu Y et al. Comparison of O-antigen gene clusters of all O-serogroups of Escherichia coli and proposal for adopting a new nomenclature for O-typing. PLoS One 2016;11:e0147434 [CrossRef][PubMed]
    [Google Scholar]
  7. Iguchi A, Iyoda S, Seto K, Nishii H, Ohnishi M et al. Six novel O genotypes from Shiga toxin-producing Escherichia coli. Front Microbiol 2016;7:765 [CrossRef][PubMed]
    [Google Scholar]
  8. Joensen KG, Tetzschner AM, Iguchi A, Aarestrup FM, Scheutz F. Rapid and easy in silico serotyping of Escherichia coli isolates by use of whole-genome sequencing data. J Clin Microbiol 2015;53:2410–2426 [CrossRef][PubMed]
    [Google Scholar]
  9. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary Genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  10. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  11. Shaheen HI, Khalil SB, Rao MR, Abu Elyazeed R, Wierzba TF et al. Phenotypic profiles of enterotoxigenic Escherichia coli associated with early childhood diarrhea in rural Egypt. J Clin Microbiol 2004;42:5588–5595 [CrossRef][PubMed]
    [Google Scholar]
  12. Rodas C, Mamani R, Blanco J, Blanco JE, Wiklund G et al. Enterotoxins, colonization factors, serotypes and antimicrobial resistance of enterotoxigenic Escherichia coli (ETEC) strains isolated from hospitalized children with diarrhea in Bolivia. Braz J Infect Dis 2011;15:132–137 [CrossRef][PubMed]
    [Google Scholar]
  13. Ansaruzzaman M, Bhuiyan NA, Begum YA, Kühn I, Nair GB et al. Characterization of enterotoxigenic Escherichia coli from diarrhoeal patients in Bangladesh using phenotyping and genetic profiling. J Med Microbiol 2007;56:217–222 [CrossRef][PubMed]
    [Google Scholar]
  14. Chen Y, Chen X, Zheng S, Yu F, Kong H et al. Serotypes, genotypes and antimicrobial resistance patterns of human diarrhoeagenic Escherichia coli isolates circulating in southeastern China. Clin Microbiol Infect 2014;20:52–58 [CrossRef][PubMed]
    [Google Scholar]
  15. Konishi N, Obata H, Monma C, Nakama A, Kai A et al. Bacteriological and epidemiological characteristics of enterotoxigenic Escherichia coli isolated in Tokyo, Japan, between 1966 and 2009. J Clin Microbiol 2011;49:3348–3351 [CrossRef][PubMed]
    [Google Scholar]
  16. Svennerholm AM, Lundgren A. Recent progress toward an enterotoxigenic Escherichia coli vaccine. Expert Rev Vaccines 2012;11:495–507 [CrossRef][PubMed]
    [Google Scholar]
  17. Dedeić-Ljubović A, Hukić M, Bekić D, Zvizdić A. Frequency and distribution of diarrhoeagenic Escherichia coli strains isolated from pediatric patients with diarrhoea in Bosnia and Herzegovina. Bosn J Basic Med Sci 2009;9:148–155[PubMed]
    [Google Scholar]
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