RT Journal Article SR Electronic(1) A1 Ríos, Paul A1 Rocha, Claudio A1 Castro, William A1 Vidal, Maria A1 Canal, Enrique A1 Bernal, Manuela A1 Reynolds, Nathanael D. A1 Tilley, Drake H. A1 Simons, Mark P.YR 2018 T1 Extensively drug-resistant (XDR) Pseudomonas aeruginosa identified in Lima, Peru co-expressing a VIM-2 metallo-β-lactamase, OXA-1 β-lactamase and GES-1 extended-spectrum β-lactamase JF JMM Case Reports, VO 5 IS 7 OP SP e005154 DO https://doi.org/10.1099/jmmcr.0.005154 PB Microbiology Society, SN 2053-3721, AB Introduction. Pseudomonas aeruginosa has the ability to acquire plasmids and other mobile genetic elements that confer resistance to antibiotics. Bacterial genes encoding different β-lactamases (bla), such as metallo-β-lactamases (MBLs) and extended-spectrum β-lactamases (ESBL), can confer resistance to multiple classes of β-lactam antibiotics. Case presentation. An 83 year old female was admitted in 2012 to the Peruvian Naval Hospital, Centro Médico Naval ‘Cirujano Mayor Santiago Távara’ (CEMENA), in Lima, Peru. A midstream urine sample was collected and sent to the local CEMENA laboratory for routine urine culture. P. aeruginosa was isolated and initial antibiotic susceptibility testing showed it to be sensitive to imipenem. The clinicians started a course of meropenem, but the patient did not improve. After 5 days, a second urine culture was performed and a P. aeruginosa was isolated again, but this time the strain showed resistance to imipenem. The treatment course was changed to fosfomycin and the patient improved. Phenotypic and molecular laboratory testing to characterize the antibiotic resistance were performed, demonstrating the presence of both MBL and ESBL genes. Conclusion. To our knowledge, this is the first report of a P. aeruginosa XDR clinical isolate that co-expresses an MBL (VIM-2), OXA-1 beta-lactamase and the ESBL (GES-1) in Peru. It is also the first report of a VIM carbapenemase in Peru., UL https://www.microbiologyresearch.org/content/journal/jmmcr/10.1099/jmmcr.0.005154