First confirmed case of nasal pythiosis in a horse in Thailand

Walaiporn Tonpitak; Watcharapol Pathomsakulwong; Chulabha Sornklien; Theerapong Krajaejun; Suppathat Wutthiwithayaphong

doi:10.1099/jmmcr.0.005136

Volume 5, Issue 1

Case Report

Open Access

First confirmed case of nasal pythiosis in a horse in Thailand

Walaiporn Tonpitak¹, Watcharapol Pathomsakulwong², Chulabha Sornklien¹, Theerapong Krajaejun³ and Suppathat Wutthiwithayaphong⁴
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Affiliations: ¹ Department of Microbiology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand ² Equine Clinic, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen campus, Nakhon Pathom, Thailand ³ Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand ⁴ Clinic for Horse, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand
*Correspondence: Walaiporn Tonpitak, [email protected]
Published: 02 January 2018 https://doi.org/10.1099/jmmcr.0.005136

Abstract

Introduction. Pythiosis is caused by Pythium insidiosum, a fungus-like organism in the class Oomycetes. It can infect humans and a variety of animal species in tropical, subtropical and some temperate regions. Cases of animal pythiosis have occurred predominantly in horses in the skin and subcutaneous tissue at the limbs and in the ventral portion of thoracoabdominal wall - lesions in the nasal region are rarely reported. Moreover, although many human pythiosis cases have been reported in Thailand, no cases of animal pythiosis in Thailand have been reported.

Case presentation. We report a case of pythiosis in a horse infected at the nasal cavity. Diagnosis was performed by zoospore formation by bait technique, immunohistochemical stain, immunochromatography and sequence analysis.

Conclusion. The sequences of rDNA were 99 % and 96 to 99 % identical to GenBank isolates of Pythium insidiosum from two Thai human patients and horses from various countries, respectively. This represents the first confirmed report of nasal equine pythiosis in Thailand.

Received: 20/09/2017
Accepted: 10/12/2017
Published Online: 02/01/2018

Keyword(s): horse , pythiosis , Pythium insidiosum and Thailand

This is an open access article under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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First confirmed case of nasal pythiosis in a horse in Thailand

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Volume 5, Issue 1

Case Report

Open Access

First confirmed case of nasal pythiosis in a horse in Thailand

Abstract

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