1887

Abstract

Pythiosis is caused by , a fungus-like organism in the class . It can infect humans and a variety of animal species in tropical, subtropical and some temperate regions. Cases of animal pythiosis have occurred predominantly in horses in the skin and subcutaneous tissue at the limbs and in the ventral portion of thoracoabdominal wall - lesions in the nasal region are rarely reported. Moreover, although many human pythiosis cases have been reported in Thailand, no cases of animal pythiosis in Thailand have been reported.

We report a case of pythiosis in a horse infected at the nasal cavity. Diagnosis was performed by zoospore formation by bait technique, immunohistochemical stain, immunochromatography and sequence analysis.

The sequences of rDNA were 99 % and 96 to 99 % identical to GenBank isolates of from two Thai human patients and horses from various countries, respectively. This represents the first confirmed report of nasal equine pythiosis in Thailand.

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/content/journal/jmmcr/10.1099/jmmcr.0.005136
2018-01-02
2020-09-25
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References

  1. Gaastra W, Lipman LJ, De Cock AW, Exel TK, Pegge RB et al. Pythium insidiosum: an overview. Vet Microbiol 2010;146:1–16 [CrossRef][PubMed]
    [Google Scholar]
  2. Supabandhu J, Fisher MC, Mendoza L, Vanittanakom N. Isolation and identification of the human pathogen Pythium insidiosum from environmental samples collected in Thai agricultural areas. Med Mycol 2008;46:41–52 [CrossRef][PubMed]
    [Google Scholar]
  3. Chaiprasert A, Krajaejun T, Pannanusorn S, Prariyachatigul C, Wanachiwanawin W et al. Pythium insidiosum Thai isolates: molecular phylogenetic analysis. Asian Biomedicine 2009;6:623–633
    [Google Scholar]
  4. Krajaejun T, Sathapatayavongs B, Pracharktam R, Nitiyanant P, Leelachaikul P et al. Clinical and epidemiological analyses of human pythiosis in Thailand. Clin Infect Dis 2006;43:569–576 [CrossRef][PubMed]
    [Google Scholar]
  5. Mendoza L, Ajello L, McGinnis MR. Infection caused by the oomycetous pathogen Pythium insidiosum. J Mycol Med 1996;6:151–164
    [Google Scholar]
  6. Souto EPF, Maia LA, Olinda RG, Galiza GJN, Kommers GD et al. Pythiosis in the nasal cavity of horses. J Comp Pathol 2016;155:126–129 [CrossRef][PubMed]
    [Google Scholar]
  7. Chareonsirisuthigul T, Khositnithikul R, Intaramat A, Inkomlue R, Sriwanichrak K et al. Performance comparison of immunodiffusion, enzyme-linked immunosorbent assay, immunochromatography and hemagglutination for serodiagnosis of human pythiosis. Diagn Microbiol Infect Dis 2013;76:42–45 [CrossRef][PubMed]
    [Google Scholar]
  8. Keeratijarut A, Karnsombut P, Aroonroch R, Srimuang S, Sangruchi T et al. Evaluation of an in-house immunoperoxidase staining assay for histodiagnosis of human pythiosis. Southeast Asian J Trop Med Public Health 2009;40:1298–1305[PubMed]
    [Google Scholar]
  9. Mendoza L, Prendas J. A method to obtain rapid zoosporogenesis of Pythium insidiosum. Mycopathologia 1988;104:59–62 [CrossRef][PubMed]
    [Google Scholar]
  10. Intaramat A, Sornprachum T, Chantrathonkul B, Chaisuriya P, Lohnoo T et al. Protein A/G-based immunochromatographic test for serodiagnosis of pythiosis in human and animal subjects from Asia and Americas. Med Mycol 2016;54:641–647 [CrossRef][PubMed]
    [Google Scholar]
  11. Lohnoo T, Jongruja N, Rujirawat T, Yingyon W, Lerksuthirat T et al. Efficiency comparison of three methods for extracting genomic DNA of the pathogenic oomycete Pythium insidiosum. J Med Assoc Thai 2014;97:342–348[PubMed]
    [Google Scholar]
  12. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Prortocols: a Guide to Methods and Applications SanDiego: Academic Press; 1990; pp.315–322
    [Google Scholar]
  13. Rujirawat T, Sridapan T, Lohnoo T, Yingyong W, Kumsang Y et al. Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment. Infect Genet Evol 2017;54:429–436 [CrossRef][PubMed]
    [Google Scholar]
  14. Dória RG, Carvalho MB, Freitas SH, Laskoski LM, Colodel EM et al. Evaluation of intravenous regional perfusion with amphotericin B and dimethylsulfoxide to treat horses for pythiosis of a limb. BMC Vet Res 2015;11:152 [CrossRef][PubMed]
    [Google Scholar]
  15. Santurio JM, Argenta JS, Schwendler SE, Cavalheiro AS, Pereira DI et al. Granulomatous rhinitis associated with Pythium insidiosum infection in sheep. Vet Rec 2008;163:276–277 [CrossRef][PubMed]
    [Google Scholar]
  16. Ubiali DG, Cruz RA, de Paula DA, Silva MC, Mendonça FS et al. Pathology of nasal infection caused by Conidiobolus lamprauges and Pythium insidiosum in sheep. J Comp Pathol 2013;149:137–145 [CrossRef][PubMed]
    [Google Scholar]
  17. Grooters AM, Whittington A, Lopez MK, Boroughs MN, Roy AF. Evaluation of microbial culture techniques for the isolation of Pythium insidiosum from equine tissues. J Vet Diagn Invest 2002;14:288–294 [CrossRef][PubMed]
    [Google Scholar]
  18. Salas Y, Márquez A, Canelón J, Perazzo Y, Colmenárez V et al. Equine pythiosis: report in crossed bred (Criole Venezuelan) horses. Mycopathologia 2012;174:511–517 [CrossRef][PubMed]
    [Google Scholar]
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