@article{mbs:/content/journal/jmmcr/10.1099/jmmcr.0.005099, author = "Saupe, Angela and Edel, Birgit and Pfister, Wolfgang and Löffler, Bettina and Ehricht, Ralf and Rödel, Jürgen", title = "Acute diarrhoea due to a Shiga toxin 2e-producing Escherichia coli O8 : H19", journal= "JMM Case Reports", year = "2017", volume = "4", number = "6", pages = "", doi = "https://doi.org/10.1099/jmmcr.0.005099", url = "https://www.microbiologyresearch.org/content/journal/jmmcr/10.1099/jmmcr.0.005099", publisher = "Microbiology Society", issn = "2053-3721", type = "Journal Article", keywords = "azithromycin", keywords = "diarrhoea", keywords = "antibiotics", keywords = "STEC infection", eid = "e005099", abstract = " Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx 2e-positive STEC of serotype O8 : H19. The strain harboured lpfA, but not eae. Conclusion. This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.", }