1887

Abstract

Introduction:

Wound infections with , a Gram-negative bacterium found in all temperate oceans, are rarely reported. However, a rising incidence of wound infections caused by requires better knowledge about this infectious agent.

Case presentation:

We report the case of a 14-year-old boy suffering from a wound infection caused by and after stepping on a sea urchin. Despite wound debridement and antibiotic therapy with cefaclor, the lesion did not heal over several weeks. After identification of the pathogens and antibiotic-susceptibility testing, antibiotic therapy was switched to ciprofloxacin, followed by trimethoprim/sulfamethoxazole. Two months after the accident the wound was re-epithelialized. Follow up after 6 months revealed a painful scar.

Conclusion:

Non-cholera vibrios like should be considered as possible causative agents in seawater-contaminated wounds. is a relevant pathogen in mixed wound infections. Early microbiological diagnosis and antibiotic-susceptibility testing is crucial to prevent therapeutic failure.

Loading

Article metrics loading...

/content/journal/jmmcr/10.1099/jmmcr.0.005074
2016-11-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jmmcr/3/6/jmmcr005074.html?itemId=/content/journal/jmmcr/10.1099/jmmcr.0.005074&mimeType=html&fmt=ahah

References

  1. Altoparlak U., Erol S., Akcay M. N., Celebi F., Kadanali A. 2004; The time-related changes of antimicrobial resistance patterns and predominant bacterial profiles of burn wounds and body flora of burned patients. Burns 30:660–664 [View Article][PubMed]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  3. Bauer A., Rørvik L. M. 2007; A novel multiplex PCR for the identification of Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus . Lett Appl Microbiol 45:371–375 [View Article][PubMed]
    [Google Scholar]
  4. Becker K., Heilmann C., Peters G. 2014; Coagulase-negative staphylococci. Clin Microbiol Rev 27:870–926 [View Article]
    [Google Scholar]
  5. CLSI 2008 Interpretive Criteria for Identification of Bacteria and Fungi by DNA Target Sequencing; Approved Guideline, MM18-A. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  6. CLSI 2015a Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; 3rd edn M45 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  7. CLSI 2015b Performance Standards for Antimicrobial Susceptibility Testing; 25th edn M100S Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  8. Bradley J. S., Jackson M. A. the Committee on Infectious Diseases 2011; The use of systemic and topical fluoroquinolones. Pediatrics 128:e1034e1045 [View Article][PubMed]
    [Google Scholar]
  9. Conrad A. 2013; Trends in vibriosis transmission among the top four Vibrio species, United States, 1988-2012. MPH thesis, Georgia State University, Atlanta, GA, USA Available at: http://scholarworks.gsu.edu/iph_theses/314
  10. French G. L., Woo M. L., Hui Y. W., Chan K. Y. 1989; Antimicrobial susceptibilities of halophilic vibrios. J Antimicrob Chemother 24:183–194 [View Article][PubMed]
    [Google Scholar]
  11. Gomez J. M., Fajardo R., Patiño J. F., Arias C. A. 2003; Necrotizing fasciitis due to Vibrio alginolyticus in an immunocompetent patient. J Clin Microbiol 41:3427–3429 [View Article][PubMed]
    [Google Scholar]
  12. Harmsen D., Dostal S., Roth A., Niemann S., Rothgänger J., Sammeth M., Albert J., Frosch M., Richter E. 2003; RIDOM: comprehensive and public sequence database for identification of Mycobacterium species. BMC Infect Dis 3:26 [View Article][PubMed]
    [Google Scholar]
  13. Ki J. S., Zhang R., Zhang W., Huang Y. L., Qian P. Y. 2009; Analysis of RNA polymerase beta subunit (rpoB) gene sequences for the discriminative power of marine Vibrio species. Microb Ecol 58:679–691 [View Article][PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  15. Li J., Yie J., Foo R. W. T., Ling J. M., Xu H., Woo N. Y. S. 1999; Antibiotic resistance and plasmid profiles of Vibrio isolates from cultured silver sea bream, Sparus sarba . Mar Pollut Bull 39:245–249 [View Article]
    [Google Scholar]
  16. Oberbeckmann S., Wichels A., Maier T., Kostrzewa M., Raffelberg S., Gerdts G. 2011; A polyphasic approach for the differentiation of environmental Vibrio isolates from temperate waters. FEMS Microbiol Ecol 75:145–162 [View Article][PubMed]
    [Google Scholar]
  17. Osaka K., Komatsuzaki M., Takahashi H., Sakano S., Okabe N. 2004; Vibrio vulnificus septicaemia in Japan: an estimated number of infections and physicians' knowledge of the syndrome. Epidemiol Infect 132:993–996 [View Article][PubMed]
    [Google Scholar]
  18. Osorio C. R., Klose K. E. 2000; A region of the transmembrane regulatory protein ToxR that tethers the transcriptional activation domain to the cytoplasmic membrane displays wide divergence among Vibrio species. J Bacteriol 182:526–528 [View Article][PubMed]
    [Google Scholar]
  19. Reilly G. D., Reilly C. A., Smith E. G., Baker-Austin C. 2011; Vibrio alginolyticus-associated wound infection acquired in British waters, Guernsey, July 2011. Euro Surveill 16:19994[PubMed]
    [Google Scholar]
  20. Schets F. M., van den Berg H. H., Demeulmeester A. A., van Dijk E., Rutjes S. A., van Hooijdonk H. J., de Roda Husman A. M. 2006; Vibrio alginolyticus infections in the Netherlands after swimming in the North Sea. Euro Surveill 11:3077
    [Google Scholar]
  21. Tarr C. L., Patel J. S., Puhr N. D., Sowers E. G., Bopp C. A., Strockbine N. A. 2007; Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination. J Clin Microbiol 45:134–140 [View Article][PubMed]
    [Google Scholar]
  22. Vezzulli L., Brettar I., Pezzati E., Reid P. C., Colwell R. R., Höfle M. G., Pruzzo C. 2012; Long-term effects of ocean warming on the prokaryotic community: evidence from the vibrios. ISME J 6:21–30 [View Article][PubMed]
    [Google Scholar]
  23. Ye L., Li R., Lin D., Zhou Y., Fu A., Ding Q., Chan E. W., Yao W., Chen S. 2016; Characterization of an IncA/C multidrug resistance plasmid in Vibrio alginolyticus . Antimicrob Agents Chemother 60:3232–3235 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmmcr/10.1099/jmmcr.0.005074
Loading
/content/journal/jmmcr/10.1099/jmmcr.0.005074
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error