1887

Abstract

Introduction:

(ZIKV) and (CHIKV) can share the same mosquito vector, and co-infections by these viruses can occur in humans. While infections with these viruses share commonalities, CHIKV is unique in causing arthritis and arthralgias that may persist for a year or more. These infections are commonly diagnosed by RT–PCR-based methods during the acute phase of infection. Even with the high specificity and sensitivity characteristic of PCR, false negatives can occur, highlighting the need for additional diagnostic methods for confirmation.

Case presentation:

On her return to the USA, a traveller to Colombia, South America developed an illness consistent with Zika, Chikungunya and/or Dengue. RT-PCR of her samples was positive only for ZIKV. However, arthralgias persisted for months, raising concerns about co-infection with CHIKV or viruses. Cell cultures inoculated with her original clinical samples demonstrated two types of cytopathic effects, and both ZIKV and CHIKV were identified in the supernatants. On phylogenetic analyses, both viruses were found to be related to strains found in Colombia.

Conclusion:

These findings highlight the need to consider CHIKV co-infection in patients with prolonged rheumatological symptoms after diagnosis with ZIKV, and the usefulness of cell culture as an amplification step for low-viremia blood and other samples.

Loading

Article metrics loading...

/content/journal/jmmcr/10.1099/jmmcr.0.005072
2016-11-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jmmcr/3/6/jmmcr005072.html?itemId=/content/journal/jmmcr/10.1099/jmmcr.0.005072&mimeType=html&fmt=ahah

References

  1. Balm M. N., Lee C. K., Lee H. K., Chiu L., Koay E. S., Tang J. W. 2012; A diagnostic polymerase chain reaction assay for Zika virus. J Med Virol 84:1501–1505 [View Article][PubMed]
    [Google Scholar]
  2. Brasil P., Calvet G. A., Siqueira A. M., Wakimoto M., de Sequeira P. C., Nobre A., Quintana M. S., Mendonça M. C., Lupi O. et al. 2016; Zika virus outbreak in Rio de Janeiro, Brazil: clinical characterization, epidemiological and virological aspects. PLoS Negl Trop Dis 10:e0004636 [View Article][PubMed]
    [Google Scholar]
  3. Calisher C. H., Karabatsos N., Dalrymple J. M., Shope R. E., Porterfield J. S., Westaway E. G., Brandt W. E. 1989; Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. J Gen Virol 70:37–43 [View Article][PubMed]
    [Google Scholar]
  4. Cardona-Ospina J. A., Villamil-Gómez W. E., Jimenez-Canizales C. E., Castañeda-Hernández D. M., Rodríguez-Morales A. J. 2015; Estimating the burden of disease and the economic cost attributable to Chikungunya, Colombia, 2014. Trans R Soc Trop Med Hyg 109:793–802 [View Article][PubMed]
    [Google Scholar]
  5. CDC 2016; Zikav virus – what clinicians need to know - conference call. Clinicians Outreach and Communication Activity (COCA) [Online]. Available at: http://emergency.cdc.gov/coca/calls/2016/callinfo_012616.asp26 (accessed September 1, 2016)
  6. Duffy M. R., Chen T. H., Hancock W. T., Powers A. M., Kool J. L., Lanciotti R. S., Pretrick M., Marfel M., Holzbauer S. et al. 2009; Zika virus outbreak on Yap Island, Federated States of Micronesia. N Engl J Med 360:2536–2543 [View Article][PubMed]
    [Google Scholar]
  7. Faye O., Faye O., Diallo D., Diallo M., Weidmann M., Sall A. A. 2013; Quantitative real-time PCR detection of Zika virus and evaluation with field-caught mosquitoes. Virol J 10:311 [View Article][PubMed]
    [Google Scholar]
  8. Fernanda Estofolete C., Terzian A. C., Parreira R., Esteves A., Hardman L., Greque G. V., Rahal P., Nogueira M. L. 2016; Clinical and laboratory profile of Zika virus infection in dengue suspected patients: A case series. J Clin Virol 81:25–30 [View Article][PubMed]
    [Google Scholar]
  9. Iovine N. M., Lednicky J., Cherabuddi K., Crooke H., White S. K., Loeb J. C., Cella E., Ciccozzi M., Salemi M., Morris J. G. 2016; Coinfection with Zika and Dengue-2 viruses in a traveler returning from Haiti, 2016: clinical presentation and Genetic analysis. Clin Infect Disciw667 [View Article][PubMed]
    [Google Scholar]
  10. Krejbich-Trotot P., Denizot M., Hoarau J. J., Jaffar-Bandjee M. C., Das T., Gasque P. 2011; Chikungunya virus mobilizes the apoptotic machinery to invade host cell defenses. FASEB J 25:314–325 [View Article][PubMed]
    [Google Scholar]
  11. Lednicky J., De Rochars V. M. B., El Badry M., Loeb J., Telisma T., Chavannes S., Anilis G., Cella E., Ciccozzi M. et al. 2016a; Mayaro virus in child with acute febrile illness, Haiti, 2015. Emerg Infect Dis 22:2000–2002 [View Article]
    [Google Scholar]
  12. Lednicky J., De Rochars V. M. B., El Badry M., Loeb J., Telisma T., Chavannes S., Anilis G., Cella E., Ciccozzi M. et al. 2016b; Zika virus outbreak in Haiti in 2014: molecular and clinical data. PLoS Negl Trop Dis 10:e0004687 [View Article]
    [Google Scholar]
  13. Nguyen L. T., Schmidt H. A., von Haeseler A., Minh B. Q. 2015; IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 32:268–274 [View Article][PubMed]
    [Google Scholar]
  14. Pacheco O., Beltrán M., Nelson C. A., Valencia D., Tolosa N., Farr S. L., Padilla A. V., Tong V. T., Cuevas E. L. et al. 2016; Zika virus disease in Colombia - preliminary report. N Engl J Med doi:10.1056/NEJMoa1604037 [View Article][PubMed]
    [Google Scholar]
  15. Sardi S. I., Somasekar S., Naccache S. N., Bandeira A. C., Tauro L. B., Campos G. S., Chiu C. Y. 2016; Coinfections of Zika and Chikungunya viruses in Bahia, Brazil, identified by metagenomic next-generation sequencing. J Clin Microbiol 54:2348–2353 [View Article][PubMed]
    [Google Scholar]
  16. Villamil-Gómez W. E., Rodríguez-Morales A. J., Uribe-García A. M., González-Arismendy E., Castellanos J. E., Calvo E. P., Álvarez-Mon M., Musso D. 2016; Zika, Dengue, and Chikungunya co-infection in a pregnant woman from Colombia. Int J Infect Dis 51:135–138 [View Article][PubMed]
    [Google Scholar]
  17. Wikan N., Smith D. R. 2016; Zika virus: history of a newly emerging arbovirus. Lancet Infect Dis 16:e119126 [View Article][PubMed]
    [Google Scholar]
  18. Wikan N., Sakoonwatanyoo P., Ubol S., Yoksan S., Smith D. R. 2012; Chikungunya virus infection of cell lines: analysis of the East, Central and South African lineage. PLoS One 7:e31102 [View Article][PubMed]
    [Google Scholar]
  19. Zambrano H., Waggoner J. J., Almeida C., Rivera L., Benjamin J. Q., Pinsky B. A. 2016; Zika virus and Chikungunya virus coinfections: a series of three cases from a single center in Ecuador. Am J Trop Med Hyg 95:894–896 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmmcr/10.1099/jmmcr.0.005072
Loading
/content/journal/jmmcr/10.1099/jmmcr.0.005072
Loading

Data & Media loading...

Supplements

Supplementary File 1

WORD

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error