1887

Abstract

Introduction:

spp. infections are uncommon. However, a few clinical cases have been reported in the literature, particularly those involving immunocompromised hosts. Advanced microbiology diagnosis techniques, such as matrix-assisted laser desorption ionization-time of flight MS (MALDI-TOF MS), have been recently introduced in clinical microbiology laboratories in order to improve microbial identification, resulting in better patient management.

Case presentation:

Here, we present a new clinical case of persistent wound infection caused by in a 64-year-old woman after a mitral valve replacement, using two MALDI-TOF-based systems for identifying this micro-organism.

Conclusion:

Both MALDI-TOF systems were able to identify spp.; thus, providing a useful tool that overcomes the current limitations of phenotypic identification associated with this micro-organism. Although the technique validation deserves additional verification, our study provides guidance about MALDI-TOF as a fast and easy method for spp. identification.

Loading

Article metrics loading...

/content/journal/jmmcr/10.1099/jmmcr.0.005067
2016-09-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/jmmcr/3/5/jmmcr005067.html?itemId=/content/journal/jmmcr/10.1099/jmmcr.0.005067&mimeType=html&fmt=ahah

References

  1. Alatoom A. A., Cazanave C. J., Cunningham S. A., Ihde S. M., Patel R.. 2012; Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol50:160–163 [CrossRef][PubMed]
    [Google Scholar]
  2. Barberis C., Almuzara M., Join-Lambert O., Ramírez M. S., Famiglietti A., Vay C.. 2014; Comparison of the Bruker MALDI-TOF mass spectrometry system and conventional phenotypic methods for identification of Gram-positive rods. PLos One9:e106303 [CrossRef]
    [Google Scholar]
  3. Bizzini A., Jaton K., Romo D., Bille J., Prod'hom G., Greub G.. 2011; Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains. J Clin Microbiol49:693–696 [CrossRef][PubMed]
    [Google Scholar]
  4. Chang J. H., Ji M., Hong H. L., Choi S. H., Kim Y. S., Chung C. H., Sung H., Kim M. N.. 2014; Sternal osteomyelitis caused by Gordonia bronchialis after open-heart surgery. Infect Chemother46:110–114 [CrossRef][PubMed]
    [Google Scholar]
  5. CLSI 2011; Susceptibility Testing of Mycobateria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard, 2nd edn, M24-A2 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  6. Conville P. S., Witebsky F. G.. 2015; Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces and other aerobic actinomycetes. In Manual of Clinical Microbiology, 11th edn. pp.504–535 Edited by Jorgensen J. H., Pfaller M. A., Carroll K. C., Funke G., Landry M. L., Richter S. S., Warnock D. W.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Guerrero Gómez, C., Casañ C., Antequera P., Candel C., Blázquez R.. 2014; Catheter-related bloodstream infection caused by Gordonia terrae in a bone-marrow transplant patient: case report and review of the literature. JMM Case Rep1:
    [Google Scholar]
  8. Hsueh P. R., Lee T. F., Du S. H., Teng S. H., Liao C. H., Sheng W. H., Teng L. J.. 2014; Bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria species. J Clin Microbiol52:2371–2379 [CrossRef][PubMed]
    [Google Scholar]
  9. Johnson J. A., Onderdonk A. B., Cosimi L. A., Yawetz S., Lasker B. A., Bolcen S. J., Brown J. M., Marty F. M.. 2011; Gordonia bronchialis bacteremia and pleural infection: case report and review of the literature. J Clin Microbiol49:1662–1666 [CrossRef][PubMed]
    [Google Scholar]
  10. Lam J. Y., Wu A. K., Leung W. S., Cheung I., Tsang C. C., Chen J. H., Chan J. F., Tse C. W., Lee R. A. et al. 2015; Gordonia species as emerging causes of continuous-ambulatory-peritoneal-dialysis-related peritonitis identified by 16S rRNA and secA1 gene sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). J Clin Microbiol53:671–676 [CrossRef][PubMed]
    [Google Scholar]
  11. Nguyen D. B., Gupta N., Abou-Daoud A., Klekamp B. G., Rhone C., Winston T., Hedberg T., Scuteri A., Evans C. et al. 2014; A polymicrobial outbreak of surgical site infections following cardiac surgery at a community hospital in Florida, 2011-2012. Am J Infect Control42:432–435 [CrossRef][PubMed]
    [Google Scholar]
  12. Richet H. M., Craven P. C., Brown J. M., Lasker B. A., Cox C. D., McNeil M. M., Tice A. D., Jarvis W. R., Tablan O. C.. 1991; A cluster of Rhodococcus (Gordona) bronchialis sternal-wound infections after coronary-artery bypass surgery. N Engl J Med324:104–109 [CrossRef][PubMed]
    [Google Scholar]
  13. Siddiqui N., Toumeh A., Georgescu C.. 2012; Tibial osteomyelitis caused by Gordonia bronchialis in an immunocompetent patient. J Clin Microbiol50:3119–3121 [CrossRef][PubMed]
    [Google Scholar]
  14. Sng L. H., Koh T. H., Toney S. R., Floyd M., Butler W. R., Tan B. H.. 2004; Bacteremia caused by Gordonia bronchialis in a patient with sequestrated lung. J Clin Microbiol42:2870–2871 [CrossRef][PubMed]
    [Google Scholar]
  15. Theel E. S., Schmitt B. H., Hall L., Cunningham S. A., Walchak R. C., Patel R., Wengenack N. L.. 2012; Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol50:3093–3095 [CrossRef][PubMed]
    [Google Scholar]
  16. Titécat M., Courcol R. J., Loı¨ez C., Wallet F.. 2014; Difficulty with Gordonia bronchialis identification by Microflex mass spectrometer in a pacemaker‐induced endocarditis. JMM Case Rep1:[CrossRef]
    [Google Scholar]
  17. Tsukamura M.. 1971; Proposal of a new genus, Gordona, for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J Gen Microbiol68:15–26 [CrossRef][PubMed]
    [Google Scholar]
  18. Vasquez M. A., Marne C., Villuendas M. C., Arazo P.. 2013; Osteomielitis esternal subaguda por Gordonia bronchialis tras cirugía cardiaca. Enferm Infecc Microbiol Clin31:559–560 [CrossRef]
    [Google Scholar]
  19. Werno A. M., Anderson T. P., Chambers S. T., Laird H. M., Murdoch D. R.. 2005; Recurrent breast abscess caused by Gordonia bronchialis in an immunocompetent patient. J Clin Microbiol43:3009–3010 [CrossRef][PubMed]
    [Google Scholar]
  20. Wright S. N., Gerry J. S., Busowski M. T., Klochko A. Y., McNulty S. G., Brown S. A., Sieger B. E., Ken Michaels P., Wallace M. R.. 2012; Gordonia bronchialis sternal wound infection in 3 patients following open heart surgery: intraoperative transmission from a healthcare worker. Infect Control Hosp Epidemiol33:1238–1241 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmmcr/10.1099/jmmcr.0.005067
Loading
/content/journal/jmmcr/10.1099/jmmcr.0.005067
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error