1887

Abstract

Introduction:

spp. colonizing the human stomach other than have been rarely described in children.

Case Presentation:

We identified an uncultured by histology and PCR in biopsies from a symptomatic child. Surprisingly, the PCR assay demonstrated the presence of the gene in this uncultured and its sequence related highly to virulence factor. The analysis of the 16S ribosomal sequence showed higher identity with uncultured spp. and the strain, respectively.

Conclusion:

This is the first report that shows the presence of in an uncultured extracted from human gastric mucosa.

Loading

Article metrics loading...

/content/journal/jmmcr/10.1099/jmmcr.0.005026
2016-03-15
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jmmcr/3/2/jmmcr005026.html?itemId=/content/journal/jmmcr/10.1099/jmmcr.0.005026&mimeType=html&fmt=ahah

References

  1. Alikhani M. Y. , Sadeghifard S. N. , Farajnia S. , Hajia M. , Aslani M. M. , Zamani A. R. . 2007; Evaluation of selective and nonselective media for isolation of Helicobacter pylori from gastric biopsy specimens. Pak J Biol Sci 10: 4156– 4159 [CrossRef] [PubMed]
    [Google Scholar]
  2. Andersen L. P. , Boye K. , Blom J. , Holck S. , Norgaard A. , Elsborg L. . 1999; Characterization of a culturable “Gastrospirillum hominis” (Helicobacter heilmannii) strain isolated from human gastric mucosa. J Clin Microbiol 37: 1069– 1076 [PubMed]
    [Google Scholar]
  3. Boyanova L. , Koumanova R. , Lazarova E. , Jelev C. . 2003; Helicobacter pylori and Helicobacter heilmannii in children. A Bulgarian study. Diagn Microbiol Infect Dis 46: 249– 252 [CrossRef] [PubMed]
    [Google Scholar]
  4. Cantet F. , Magras C. , Marais A. , Federighi M. , Mégraud F. . 1999; Helicobacter species colonizing pig stomach: molecular characterization and determination of prevalence. Appl Environ Microbiol 65: 4672– 4676 [PubMed]
    [Google Scholar]
  5. Cover T. L. , Blanke S. R. . 2005; Helicobacter pylori VacA, a paradigm for toxin multifunctionality. Nat Rev Microbiol 3: 320– 332 [CrossRef] [PubMed]
    [Google Scholar]
  6. De Groote D. , van Doorn L. J. , Ducatelle R. , Verschuuren A. , Haesebrouck F. , Quint W. G. , Jalava K. , Vandamme P. . 1999; Candidatus Helicobacter suis’, a gastric helicobacter from pigs, and its phylogenetic relatedness to other gastrospirilla. Int J Syst Bacteriol 49: 1769– 1777 [CrossRef] [PubMed]
    [Google Scholar]
  7. Dieterich C. , Wiesel P. , Neiger R. , Blum A. , Corthésy-Theulaz I. . 1998; Presence of multiple “Helicobacter heilmannii” strains in an individual suffering from ulcers and in his two cats. J Clin Microbiol 36: 1366– 1370 [PubMed]
    [Google Scholar]
  8. Haesebrouck F. , Pasmans F. , Flahou B. , Chiers K. , Baele M. , Meyns T. , Decostere A. , Ducatelle R. . 2009; Gastric helicobacters in domestic animals and nonhuman primates and their significance for human health. Clin Microbiol Rev 22: 202– 223 [CrossRef] [PubMed]
    [Google Scholar]
  9. Haesebrouck F. , Pasmans F. , Flahou B. , Smet A. , Vandamme P. , Ducatelle R. . 2011; Non-Helicobacter pylori Helicobacter species in the human gastric mucosa: a proposal to introduce the terms H. heilmannii sensu lato and sensu stricto. Helicobacter 16: 339– 340 [CrossRef] [PubMed]
    [Google Scholar]
  10. Iwanczak B. , Gosciniak G. , Iwanczak F. , Grabinska M. . 2006; Helicobacter heilmannii infection in gastroduodenal diseases in children. Gastroenterol Pol 13: 301– 303
    [Google Scholar]
  11. Iwanczak B. , Biernat M. , Iwanczak F. , Grabinska J. , Matusiewicz K. , Gosciniak G. . 2012; The clinical aspects of Helicobacter heilmannii infection in children with dyspeptic symptoms. J Physiol Pharmacol 63: 133– 136 [PubMed]
    [Google Scholar]
  12. Jalava K. , On S. L. , Vandamme P. A. , Happonen I. , Sukura A. , Hänninen M. L. . 1998; Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa. Appl Environ Microbiol 64: 3998– 4006 [PubMed]
    [Google Scholar]
  13. Kato S. , Ozawa K. , Sekine H. , Ohyauchi M. , Shimosegawa T. , Minoura T. , Iinuma K. . 2005; Helicobacter heilmannii infection in a child after successful eradication of Helicobacter pylori: case report and review of literature. J Gastroenterol 40: 94– 97 [CrossRef] [PubMed]
    [Google Scholar]
  14. Kumar S. , Kumar A. , Dixit V. K. . 2008; Direct detection and analysis of vacA genotypes and cagA gene of Helicobacter pylori from gastric biopsies by a novel multiplex polymerase chain reaction assay. Diagn Microbiol Infect Dis 62: 366– 373 [CrossRef] [PubMed]
    [Google Scholar]
  15. Matsumoto T. , Kawakubo M. , Shiohara M. , Kumagai T. , Hidaka E. , Yamauchi K. , Oana K. , Matsuzawa K. , Ota H. , Kawakami Y. . 2009; Phylogeny of a novel “Helicobacter heilmannii” organism from a Japanese patient with chronic gastritis based on DNA sequence analysis of 16S rRNA and urease genes. J Microbiol 47: 201– 207 [CrossRef] [PubMed]
    [Google Scholar]
  16. McClain M. S. , Cao P. , Iwamoto H. , Vinion-Dubiel A. D. , Szabo G. , Shao Z. , Cover T. L. . 2001; A 12-amino-acid segment, present in type s2 but not type s1 Helicobacter pylori VacA proteins, abolishes cytotoxin activity and alters membrane channel formation. J Bacteriol 183: 6499– 6508 [CrossRef] [PubMed]
    [Google Scholar]
  17. Mention K. , Michaud L. , Guimber D. , Martin De Lasalle E. , Vincent P. , Turck D. , Gottrand F. . 1999; Characteristics and prevalence of Helicobacter heilmannii infection in children undergoing upper gastrointestinal endoscopy. J Pediatr Gastroenterol Nutr 29: 533– 539 [CrossRef] [PubMed]
    [Google Scholar]
  18. Monno R. , Margiotta M. , Ierardi E. , De Giglio I. , Fumarola L. , Burattini O. , Di Leo A. , Pisani A. , Panella C. , Francavilla A. . 2006; Helicobacter heilmannii infection of the antrum and duodenal bulb. Clin Microbiol Newsl 28: 37– 40 [CrossRef]
    [Google Scholar]
  19. Morgner A. , Bayerdörffer E. , Meining A. , Stolte M. , Kroher G. . 1995; Helicobacter heilmannii and gastric cancer. Lancet 346: 511– 512 [CrossRef] [PubMed]
    [Google Scholar]
  20. Murano A. , Miyake M. , Kato J. , Tanzawa H. , Takeo K. , Noda M. . 1999; Enhancement of the growth of Helicobacter pylori in Brucella broth by hydrogen peroxide. Microbiol Immunol 43: 1009– 1015 [CrossRef] [PubMed]
    [Google Scholar]
  21. Neiger R. , Dieterich C. , Burnens A. , Waldvogel A. , Corthésy-Theulaz I. , Halter F. , Lauterburg B. , Schmassmann A. . 1998; Detection and prevalence of Helicobacter infection in pet cats. J Clin Microbiol 36: 634– 637 [PubMed]
    [Google Scholar]
  22. O'Rourke J. , Solnick J. V. , Lee A. , Tompkins L. S. . 1992; Helicobacter heilmannii (previously Gastrospirillum), a new species of Helicobacter in humans and animals. Irish J Med Sci 161: 31 [CrossRef]
    [Google Scholar]
  23. O'Rourke J. L. , Solnick J. V. , Neilan B. A. , Seidel K. , Hayter R. , Hansen L. M. , Lee A. . 2004; Description of ‘Candidatus Helicobacter heilmannii’ based on DNA sequence analysis of 16S rRNA and urease genes. Int J Syst Evol Microbiol 54: 2203– 2211 [CrossRef] [PubMed]
    [Google Scholar]
  24. Papini E. , de Bernard M. , Milia E. , Bugnoli M. , Zerial M. , Rappuoli R. , Montecucco C. . 1994; Cellular vacuoles induced by Helicobacter pylori originate from late endosomal compartments. Proc Natl Acad Sci U S A 91: 9720– 9724 [CrossRef] [PubMed]
    [Google Scholar]
  25. Pradhan S. B. . 2009; Study of Helicobacter hepaticus in gallbladders with cholelithiasis and its sensitivity pattern. Kathmandu Univ Med J 7: 125– 128 [PubMed] [CrossRef]
    [Google Scholar]
  26. Priestnall S. L. , Wiinberg B. , Spohr A. , Neuhaus B. , Kuffer M. , Wiedmann M. , Simpson K. W. . 2004; Evaluation of “Helicobacter heilmannii” subtypes in the gastric mucosas of cats and dogs. J Clin Microbiol 42: 2144– 2151 [CrossRef] [PubMed]
    [Google Scholar]
  27. Qualia C. M. , Katzman P. J. , Brown M. R. , Kooros K. . 2007; A report of two children with Helicobacter heilmannii gastritis and review of the literature. Pediatr Dev Pathol 10: 391– 394 [CrossRef] [PubMed]
    [Google Scholar]
  28. Solnick J. V. , O'Rourke J. , Lee A. , Paster B. J. , Dewhirst F. E. , Tompkins L. S. . 1993; An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis 168: 379– 385 [CrossRef] [PubMed]
    [Google Scholar]
  29. Sýkora J. , Hejda V. , Varvarovská J. , Stozický F. , Siala K. , Schwarz J. . 2004; Helicobacter heilmannii gastroduodenal disease and clinical aspects in children with dyspeptic symptoms. Acta Paediatr 93: 707– 709 [CrossRef] [PubMed]
    [Google Scholar]
  30. van Loon S. , Bart A. , den Hertog E. J. , Nikkels P. G. , Houwen R. H. , De Schryver J. E. , Oudshoorn J. H. . 2003; Helicobacter heilmannii gastritis caused by cat to child transmission. J Pediatr Gastroenterol Nutr 36: 407– 409 [CrossRef] [PubMed]
    [Google Scholar]
  31. Vermoote M. , Vandekerckhove T. T. , Flahou B. , Pasmans F. , Smet A. , De Groote D. , Van Criekinge W. , Ducatelle R. , Haesebrouck F. . 2011; Genome sequence of Helicobacter suis supports its role in gastric pathology. Vet Res 42: 51 [CrossRef] [PubMed]
    [Google Scholar]
  32. Yakoob J. , Abbas Z. , Khan R. , Naz S. , Ahmad Z. , Islam M. , Awan S. , Jafri F. , Jafri W. . 2012; Prevalence of non Helicobacter pylori species in patients presenting with dyspepsia. BMC Gastroenterol 12: 3 [CrossRef] [PubMed]
    [Google Scholar]
  33. Yang H. , Li X. , Xu Z. , Zhou D. . 1995; Helicobacter heilmannii” infection in a patient with gastric cancer. Dig Dis Sci 40: 1013– 1014 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmmcr/10.1099/jmmcr.0.005026
Loading
/content/journal/jmmcr/10.1099/jmmcr.0.005026
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error