Rapidly growing mycobacteria (RGM) are ubiquitous and are usually considered as saprophytes, and have been recovered from the environment, particularly in dust, watery soil and water distribution systems. However, is a rare causative agent of ocular infection.

Case presentation:

We report a case of in a 44‐year‐old female with signs and symptoms of a corneal ulcer. We carried out PCR‐based DNA sequencing targeting the gene for the identification of . To confirm the identification, we also performed PCR‐based RFLP targeting the gene and PCR‐based DNA sequencing targeting the internal transcribed spacer region, which showed 97 % nucleotide identity with .


To the best of our knowledge, this is the first study in India to report the detection of from a corneal biopsy.


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  1. Adekambi T. , Reynaud‐Gaubert M. , Greub G. , Gevaudan M.J. , La Scola B. , Raoult D. , Drancourt M. . ( 2004; ). Amoebal coculture of “Mycobacterium massiliense” sp. from the sputum of a patient with hemoptoic pneumonia. . J Clin Microbiol 42:, 5493–5501.[CrossRef]
    [Google Scholar]
  2. Kim H.Y. , Yun Y.J. , Park C.G. , Lee D.H. , Cho Y.K. , Park B.J. , Joo S.I. , Kim E.C. , Hur Y.J. . other authors ( 2007; ). Outbreak of Mycobacterium massiliense infection associated with intramuscular injections. . J Clin Microbiol 45:, 3127–3130.[CrossRef]
    [Google Scholar]
  3. Simmon K.E. , Pounder J.I. , Greene J.N. , Walsh F. , Anderson C.M. , Cohen S. , Petti C.A. . ( 2007; ). Identification of an emerging pathogen, Mycobacterium massiliense, by rpoB sequencing of clinical isolates collected in the United States. . J Clin Microbiol 45:, 1978–1980.[CrossRef]
    [Google Scholar]
  4. Telenti A. , Marchesi F. , Balz M. , Bally F. , Bottger E.C. , Bodmer T. . ( 1993; ). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. . J Clin Microbiol 31:, 175–178.
    [Google Scholar]
  5. Therese K.L. , Jayanthi U. , Madhavan H.N. . ( 2005; ). Application of nested polymerase chain reaction (nPCR) using MPB 64 gene primers to detect M. . . tuberculosis DNA in clinical specimens from extrapulmonary tuberculosis patients. Indian J Med Res 122:, 165–170.
    [Google Scholar]
  6. Viana‐Niero C. , Lima K.V.B. , Lopes M.L. , Rabello M.C. , da S. , Marsola L.R. , Brilhante V.C.R. , Durham A.M. , Leão S.C. . ( 2008; ). Molecular characterization of Mycobacterium massiliense and Mycobacterium bolletii in isolates collected from outbreaks of infections after laparoscopic surgeries and cosmetic procedures. . J Clin Microbiol 46:, 850–855.[CrossRef]
    [Google Scholar]
  7. Wang J.Y. , Lee L.N. , Chou C.S. , Huang C.Y. , Wang S.K. , Lai H.C. , Hsueh P.R. , Luh K.T. . ( 2004; ). Performance assessment of a nested‐PCR assay (the RAPID BAP‐MTB) and the BD ProbeTec ET system for detection of Mycobacterium tuberculosis in clinical specimens. . J Clin Microbiol 42:, 4599–4603.[CrossRef]
    [Google Scholar]

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