1887

Abstract

Introduction:

Rapidly growing mycobacteria (RGM) are ubiquitous and are usually considered as saprophytes, and have been recovered from the environment, particularly in dust, watery soil and water distribution systems. However, is a rare causative agent of ocular infection.

Case presentation:

We report a case of in a 44‐year‐old female with signs and symptoms of a corneal ulcer. We carried out PCR‐based DNA sequencing targeting the gene for the identification of . To confirm the identification, we also performed PCR‐based RFLP targeting the gene and PCR‐based DNA sequencing targeting the internal transcribed spacer region, which showed 97 % nucleotide identity with .

Conclusion:

To the best of our knowledge, this is the first study in India to report the detection of from a corneal biopsy.

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2014-12-01
2020-04-04
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References

  1. Adekambi T., Reynaud‐Gaubert M., Greub G., Gevaudan M.J., La Scola B., Raoult D., Drancourt M.. ( 2004;). Amoebal coculture of “Mycobacterium massiliense” sp. from the sputum of a patient with hemoptoic pneumonia. J Clin Microbiol42:5493–5501[CrossRef]
    [Google Scholar]
  2. Kim H.Y., Yun Y.J., Park C.G., Lee D.H., Cho Y.K., Park B.J., Joo S.I., Kim E.C., Hur Y.J.. other authors( 2007;). Outbreak of Mycobacterium massiliense infection associated with intramuscular injections. J Clin Microbiol45:3127–3130[CrossRef]
    [Google Scholar]
  3. Simmon K.E., Pounder J.I., Greene J.N., Walsh F., Anderson C.M., Cohen S., Petti C.A.. ( 2007;). Identification of an emerging pathogen, Mycobacterium massiliense, by rpoB sequencing of clinical isolates collected in the United States. J Clin Microbiol45:1978–1980[CrossRef]
    [Google Scholar]
  4. Telenti A., Marchesi F., Balz M., Bally F., Bottger E.C., Bodmer T.. ( 1993;). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol31:175–178
    [Google Scholar]
  5. Therese K.L., Jayanthi U., Madhavan H.N.. ( 2005;). Application of nested polymerase chain reaction (nPCR) using MPB 64 gene primers to detect M. . tuberculosis DNA in clinical specimens from extrapulmonary tuberculosis patients. Indian J Med Res122:165–170
    [Google Scholar]
  6. Viana‐Niero C., Lima K.V.B., Lopes M.L., Rabello M.C., da S., Marsola L.R., Brilhante V.C.R., Durham A.M., Leão S.C.. ( 2008;). Molecular characterization of Mycobacterium massiliense and Mycobacterium bolletii in isolates collected from outbreaks of infections after laparoscopic surgeries and cosmetic procedures. J Clin Microbiol46:850–855[CrossRef]
    [Google Scholar]
  7. Wang J.Y., Lee L.N., Chou C.S., Huang C.Y., Wang S.K., Lai H.C., Hsueh P.R., Luh K.T.. ( 2004;). Performance assessment of a nested‐PCR assay (the RAPID BAP‐MTB) and the BD ProbeTec ET system for detection of Mycobacterium tuberculosis in clinical specimens. J Clin Microbiol42:4599–4603[CrossRef]
    [Google Scholar]
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