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Abstract

Introduction:

Ranavirus infection is associated with mass die‐off and population decline in amphibians worldwide, and is listed by the World Organization for Animal Health (OIE) as a notifiable disease under the Aquatic Animal Health Code. Work is ongoing to control the spread of ranavirus, because this agent is considered an emerging pathogen of amphibians. In Japan, ranaviral diseases have been detected at each episode of die‐off of wild bullfrog juveniles since 2008. However, ranaviral disease has not been found in captive anurans.

Case presentation:

Epidemic and lethal ranaviral disease in captive and species imported from the Netherlands is reported. Poison dart frogs of three genera were imported from the Netherlands in March 2012. Disease and death were noted approximately 10 days after importation. Fifty‐three adults of five species died in about 1 month, including frogs that had been kept previously. Microscopic examination revealed necrosis and intracytoplasmic basophilic inclusion bodies in the parenchyma of multiple organs. Electron microscopy showed cytoplasmic ranavirus‐like particles within haematopoietic cells of the kidney. These particles were icosahedral, with a diameter of approximately 144 nm. Thirty‐three of 41 dead frogs showed positive PCR results for the major capsid protein gene of . The sequence obtained from five frogs was identical and did not match the registered sequence of any ranavirus.

Conclusion:

This is the first report, to our knowledge, of ranaviral disease in imported anurans of the family Dendrobatidae and genera and in Japan.

Keyword(s): frog , infectious diseases and ranavirus
  • This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/).
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/content/journal/jmmcr/10.1099/jmmcr.0.001198
2014-09-01
2024-03-28
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References

  1. Cunningham A.A., Langton T.E., Bennett P.M., Lewin J.F., Drury S.E., Gough R.E., Macgregor S.K. ( 1996). Pathological and microbiological findings from incidents of unusual mortality of the common frog (Rana temporaria). Philos Trans R Soc Lond B, Biol Sci 351:1539–1557 [CrossRef]
    [Google Scholar]
  2. Goka K., Yokoyama J., Une Y., Kuroki T., Suzuki K., Nakahara M., Kobayashi A., Inaba S., Mizutani T., Hyatt A.D. ( 2009). Amphibian chytridiomycosis in Japan: distribution, haplotypes and possible route of entry into Japan. Mol Ecol 18:4757–4774 [CrossRef]
    [Google Scholar]
  3. Kik M., Martel A., Sluijs A.S., Pasmans F., Wohlsein P., Gröne A., Rijks J.M. ( 2011). Ranavirus‐associated mass mortality in wild amphibians, The Netherlands, 2010: a first report. Vet J 190:284–286 [CrossRef]
    [Google Scholar]
  4. Kik M., Stege M., Boonyarittichaikij R., van Asten A. ( 2012). Concurrent ranavirus and Batrachochytrium dendrobatidis infection in captive frogs (Phyllobates and Dendrobates species), The Netherlands, 2012: a first report. Vet J 194:247–249 [CrossRef]
    [Google Scholar]
  5. Mao J., Hedrick R.P., Chinchar V.G. ( 1997). Molecular characterization, sequence analysis, and taxonomic position of newly isolated fish iridoviruses. Virology 229:212–220 [CrossRef]
    [Google Scholar]
  6. Miller D.L., Rajeev S., Brookins M., Cook J., Whittington L., Baldwin C.A. ( 2008). Concurrent infection with ranavirus, Batrachochytrium dendrobatidis, and Aeromonas in a captive anuran colony. J Zoo Wildl Med 39:445–449 [CrossRef]
    [Google Scholar]
  7. Miller D., Gray M., Storfer A. ( 2011). Ecopathology of ranaviruses infecting amphibians. Viruses 3:2351–2373 [CrossRef]
    [Google Scholar]
  8. OIE( 2012). Aquatic Animal Health Code. http://www.oie.int/doc/ged/D7821.PDF
    [Google Scholar]
  9. Pasmans F., Blahak S., Martel A., Pantchev N., Zwart P. ( 2008). Ranavirus‐associated mass mortality in imported red tailed knobby newts (Tylototriton kweichowensis): a case report. Vet J 176:257–259 [CrossRef]
    [Google Scholar]
  10. Picco A.M., Collins J.P. ( 2008). Amphibian commerce as a likely source of pathogen pollution. Conserv Biol 22:1582–1589 [CrossRef]
    [Google Scholar]
  11. Schloegel L.M., Daszak P., Cunningham A.A., Speare R., Hill B. ( 2010). Two amphibian diseases, chytridiomycosis and ranaviral disease, are now globally notifiable to the World Organization for Animal Health (OIE): an assessment. Dis Aquat Org 92:101–108 [CrossRef]
    [Google Scholar]
  12. Une Y., Sakuma A., Matsueda H., Nakai K., Murakami M. ( 2009). Ranavirus outbreak in North American bullfrogs (Rana catesbeiana), Japan, 2008. Emerging Infect Dis 15:1146–1147 [CrossRef]
    [Google Scholar]
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