We report Aspergillus antigen and antibody responses in a case of multiple invasive fungal diseases.
Case presentation:
The patient, a double‐lung transplant recipient, had candidaemia and invasive pulmonary aspergillosis with cerebral involvement. The follow‐up of the serum Aspergillus galactomannan antigen by ELISA showed a level increase that correlated with the patient’s aggravation. It was retrospectively completed by kinetics analysis using different anti‐Aspergillus antibody assays (ELISA and Western blotting).
Conclusion:
A balance between the Aspergillus antigen and anti‐Aspergillus antibodies was shown using different anti‐Aspergillus antibody assays. The results of these two antibody techniques appeared rather congruent.
AntinoriS.,
CorbellinoM.,
MeroniL.,
RestaF.,
SollimaS.,
TonoliniM.,
TortoranoA.M.,
MilazzoL.,
BelloL.other authors.(2013).Aspergillus meningitis: a rare clinical manifestation of central nervous system aspergillosis. Case report and review of 92 cases. J Infect 66:218–238[CrossRef]
De PauwB.,
WalshT.J.,
DonnellyJ.P.,
StevensD.A.,
EdwardsJ.E.,
CalandraT.,
PappasP.G.,
MaertensJ.,
LortholaryO.other authors.(2008). Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis 46:1813–1821[CrossRef]
HerbrechtR.,
Letscher‐BruV.,
OpreaC.,
LioureB.,
WallerJ.,
CamposF.,
VillardO.,
LiuK‐L.,
Natarajan‐AméS.other authors.(2002).Aspergillus galactomannan detection in the diagnosis of invasive aspergillosis in cancer patients. J Clin Oncol 20:1898–1906[CrossRef]
KumeH.,
YamazakiT.,
ToganoT.,
AbeM.,
TanumaH.,
KawanaS.,
OkudairaM.(2011). Epidemiology of visceral mycoses in autopsy cases in Japan: comparison of the data from 1989, 1993, 1997, 2001, 2005 and 2007 in Annual of Pathological Autopsy Cases in Japan
. Med Mycol J 52:117–127[CrossRef]
MaertensJ.,
VerhaegenJ.,
LagrouK.,
Van EldereJ.,
BoogaertsM.(2001). Screening for circulating galactomannan as a noninvasive diagnostic tool for invasive aspergillosis in prolonged neutropenic patients and stem cell transplantation recipients: a prospective validation. Blood 97:1604–1610[CrossRef]
Mennink‐KerstenM.A.S.H.,
DonnellyJ.P.,
VerweijP.E.(2004). Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis. Lancet Infect Dis 4:349–357[CrossRef]
MiceliM.H.,
GrazziuttiM.L.,
WoodsG.,
ZhaoW.,
KocogluM.H.,
BarlogieB.,
AnaissieE.(2008). Strong correlation between serum Aspergillus galactomannan index and outcome of aspergillosis in patients with hematological cancer: clinical and research implications. Clin Infect Dis 46:1412–1422[CrossRef]
PenackO.,
RempfP.,
GrafB.,
BlauI.W.,
ThielE.(2008).Aspergillus galactomannan testing in patients with long‐term neutropenia: implications for clinical management. Ann Oncol ESMO 19:984–989[CrossRef]
PiensM.A.,
ThiébautA.,
BilgerK.,
De MonbrisonF.,
MichalletM.,
PicotS.(2004). Intérêt de la détection d’antigène aspergillaire dans le liquide céphalo‐rachidien en cas de suspicion d’aspergillose cérébrale. J Mycol Med 14:28–33
SendidB.,
PoirotJ.L.,
TabouretM.,
BonninA.,
CaillotD.,
CamusD.,
PoulainD.(2002). Combined detection of mannanaemia and antimannan antibodies as a strategy for the diagnosis of systemic infection caused by pathogenic Candida species. J Med Microbiol 51:433–442
SheppardD.C.,
MarrK.A.,
FredricksD.N.,
ChiangL.Y.,
DoedtT.,
FillerS.G.(2006). Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis. Clin Microbiol Infect 12:376–380[CrossRef]
VerweijP.E.(2009). Galactomannan and anti‐Aspergillus antibodies detection for the diagnosis of invasive aspergillosis. In Aspergillus fumigatus and AspergillosisLatgéJ P,
SteinbachW J.
Washington, DC: ASM Press;
WoodsG.,
MiceliM.H.,
GrazziuttiM.L.,
ZhaoW.,
BarlogieB.,
AnaissieE.(2007). Serum Aspergillus galactomannan antigen values strongly correlate with outcome of invasive aspergillosis: a study of 56 patients with hematologic cancer. Cancer 110:830–834[CrossRef]