1887

Journal of Medical Microbiology logo - Volume 63, Issue 12, 2014

Volume 63, Issue 12

Volume 63, Issue 12, 2014

  • Veterinary microbiology

      • Cross-protective effect of a novel multi-antigen-chimeric vaccine against and infection in mice

        https://doi.org/10.1099/jmm.0.073593-0

        Staphylococcal and streptococcal species are the most common pathogens that cause bovine mastitis. Induction of a broad-spectrum protective immunity against staphylococci and streptococci by combining multiple antigens into a single vaccine is highlighted. To develop a universal vaccine candidate, a GapC-tIsdB-TRAP (GIT) construct was generated. The GIT contained the truncated GapC from , and truncated IsdB and full-length TRAP from . The humoral and cellular immune responses elicited by GIT were evaluated in mice. Antibody levels against GIT displayed a consistent tendency with antibody levels against GapC, IsdB and TRAP. The level of IFN-γ was higher in the GIT group than in the IsdB group (<0.05), and the level of IL-4 was higher in the GIT group than in the GapC or TRAP groups (<0.05). The GIT group showed an improved protection against in comparison with GapC group. A significant difference in challenge test was detected between the GIT group and the IsdB or TRAP groups (<0.05) in per cent survival of mice, and a synergistic immunoprotection against or was produced in the GIT group. These results suggested that the GIT would be a promising common vaccine candidate against and .

Top
  • Models of infection

      • Murine model of gastrointestinal infection

        https://doi.org/10.1099/jmm.0.079939-0

        is a spore-forming micro-organism responsible for foodborne illness. In this study, we focus on the host response following intragastric challenge with a pathogenic strain (B10502) isolated from a foodborne outbreak. C57BL/6J female mice were infected by gavage with strain B10502. Controls were administered with PBS. Infection leads to significant modification in relevant immune cells in the spleen, Peyer's patches (PP) and mesenteric lymph nodes (MLN). These findings correlated with an increase in the size of PP as compared with uninfected controls. Histological studies showed that infection increased the ratio of intestinal goblet cells and induces mononuclear cell infiltrates in spleen at 5 days post-infection. Evaluation of cytokine mRNA expression demonstrated a significant increase in IFN-γ in MLN after 2 days of infection. The present work demonstrates that infection of mice with vegetative is self-limited. Our findings determined relevant cell populations that were involved in the control of the pathogen through modification of the ratio and/or activation.

    • Whole glucan particles as a vaccine against murine aspergillosis

      https://doi.org/10.1099/jmm.0.079681-0

      Vaccination with heat-killed (HKY) protects against experimental infection by pathogenic fungi of five genera. Here we tested whether purified cell wall β-glucan could induce protection against systemic aspergillosis. CD-1 mice were given three weekly vaccine doses subcutaneously prior to intravenous infection with . Mice received PBS, 2.5 mg HKY, whole glucan particles (WGP), WGP conjugated to BSA (0.06 to 12 mg per dose), a soluble medium molecular mass (MMW) β-glucan alone or MMW–BSA (≤24 mg per dose). Survival and c.f.u. were determined, and cytokine induction and anti-β-glucan antibodies were assessed in vaccinated mice. Neither soluble MMW glucan, nor MMW–BSA was effective. HKY protected in two studies (survival and c.f.u. were reduced in brain and kidney organs, <0.004). Six or 12 mg WGP or WGP–BSA prolonged survival (≤0.004) and reduced c.f.u. in each organ (≤0.015) in both experiments; 0.6 mg WGP or WGP–BSA prolonged survival (≤0.015) and reduced c.f.u. (≤0.015) in one experiment. Cytokine profiles in serum and bronchoalveolar lavage from uninfected vaccinated mice showed an innate and adaptive immune profile (i.e. upregulation of colony stimulating factors, interferons, TNF-α, chemokines such as MCP-1, MIP-1α, RANTES and KC, and Th17-activating cytokines such as IL-6, IL-1β, IL-17). No anti-β-glucan antibodies were in the sera, suggesting an adaptive T cell-mediated, not a B cell-mediated, protective response. Vaccination with WGP or WGP–BSA proved protective against systemic aspergillosis, equivalent to that of HKY, supporting the potential of particulate β-glucans, alone or conjugated, as vaccines against aspergillosis.

Top
◄ 1 2
20 | 50 | 100 per page

Volumes and issues

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error