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Volume 5,
Issue 1,
1972
Volume 5, Issue 1, 1972
- Articles
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Cetrimide-Nalidixic Acid Agar As A Selective Medium For Pseudomonas Aeruginosa
More LessSummaryA series of 5358 consecutive swabs from burns were inoculated in duplicate on 0·03 per cent. cetrimide agar and on an agar medium containing 0·02 per cent. cetrimide with 15 µg per ml nalidixic acid. A larger number of swabs yielded Ps. aeruginosa and a smaller number yielded growth of other bacteria on cetrimide-nalidixic acid than on cetrimide agar.
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An Electron-Microscope Study Of Intracerebral Infection Of Mice With Low-Virulence Bordetella Pertussis
More LessSummaryAn electron-microscope study of experimentally infected mouse brain after intracerebral challenge with a low-virulence strain of Bordetella pertussis has shown the site of infection to be extracellular and between the microvilli of the ependymal cells. The types of local cells reacting in response to the infection were the ependymal cells and the underlying neuroglia, the latter reaching the ventricular space between the ependymal cells. Evidence of phagocytosis by the two different cell-types was found and there was evidence of subsequent bacterial destruction within these cells.
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Colicine Typing Of Escherichia Coli In Pigs
More LessSummaryThe effect of cultural conditions on the size of the zone of inhibition surrounding growth of colicine-producing organisms was examined. The size of the zone was not altered by the addition of 5 per cent. bovine blood to tryptic soy agar, but was slightly increased by raising the temperature of incubation from 37° to 40°C and substantially increased by extending incubation of producer organisms from 24 to 48 hr. A colicine typing system based on colicine production by field strains of E. coli was evolved and compared with serological typing in epidemiological investigations of E. coli on pigs. Colicine-producing isolates belonging to particular serological types generally belonged to the same colicine type. However, isolates belonging to a number of serological types frequently belonged to the same colicine type. A colicine typing system based on the sensitivity of field strains of E. coli to nine standard colicinogenic strains was too unreliable to serve as the basis for a colicine typing system.
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